Finding Chimeras: a Bioinformatics Strategy for Identification of Cross-linked Peptides
Finding Chimeras: a Bioinformatics Strategy for Identification of Cross-linked Peptides
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DOI：
10.1074/mcp.m800555-mcp200
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发表时间：
2010-01-01
影响因子：
7
通讯作者：
Chalkley, Robert J.
中科院分区：
文献类型：
--
作者：
Chu, Feixia;Baker, Peter R.;Chalkley, Robert J.
化学交联，随后确定的交联残基，是一个强大的方法来探测的拓扑结构和相互作用的蛋白质组装表面。在这项工作中，我们展示了一种新的生物信息学方法，使用多个程序模块的软件包“蛋白质勘探者”，极大地促进了化学交联研究中的交联肽的发现。给出了这种方法已被用于定义异二聚体和同二聚体蛋白质复合物中的接口，两者都提供了与晶体结构密切一致的结果，验证了该方法的可靠性的例子。Molecular & Cellular Proteomics 9：25-31，2010.
Chemical cross-linking, followed by identification of the cross-linked residues, is a powerful approach to probe the topologies and interacting surfaces of protein assemblies. In this work, we demonstrate a new bioinformatics approach using multiple program modules within the software package "Protein Prospector" that greatly facilitates the discovery of cross-linked peptides in chemical cross-linking studies. Examples are given for how this approach has been used for defining interfaces in heterodimeric and homodimeric protein complexes, both of which provide results in close agreement with crystal structures, verifying the reliability of the approach. Molecular & Cellular Proteomics 9: 25-31, 2010.