PRESENCE AND CHARACTERIZATION OF GLYCOLIPID SULFOTRANSFERASE IN HUMAN CANCER SERUM
PRESENCE AND CHARACTERIZATION OF GLYCOLIPID SULFOTRANSFERASE IN HUMAN CANCER SERUM
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DOI：
10.1111/j.1432-1033.1990.tb15490.x
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发表时间：
1990-04-30
期刊：
影响因子：
--
通讯作者：
ATSUTA, T
中科院分区：
文献类型：
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作者：
GASA, S;CASL, MT;ATSUTA, T
磺基转移酶可催化半乳糖神经酰胺 (GalCer) 碳水化合物的硫酸化，位于细胞高尔基体膜上，在人血清中检测其活性。为此，将有机溶剂添加到含有GalCer作为受体和磷酸腺苷磷酸[35S]-硫酸盐作为硫酸盐供体的孵育反应混合物中，以将合成的硫脂从血清蛋白中解离。随后在阴离子交换柱上分离磺脂。通过这个过程，发现人血清含有磺基转移酶活性。血清酶被Mn2+激活。 GalCer 和“活性硫酸盐”的酶的 Km 值分别为 4.6 μM 和 5.2 μM。在癌症患者的血清中测定了酶活性。肾细胞癌患者的血清活性（平均值 .+-. SE, 0.27 .+-. 0.027 pmol .cntdot. .mu.l-1 .cntdot. h-1）已被证明升高，与正常对照（平均值 .+-. SE, 0.18 .+-. 0.0014）相比显着升高（P < 0.005） pmol.cntdot..mu.l-1.cntdot.h-1) 和其他泌尿系统肿瘤的检查。
Sulfotransferase, which catalyzes sulfation of the carbohydrate of galactosylceramide (GalCer) and is localized in the Golgi membrane of cells, was assayed for activity in human serum. To do this, an organic solvent was added to the incubated reaction mixture containing GalCer as an acceptor and phosphoadenosine phospho[35S]-sulfate as a donor of sulfate to dissociate the synthesized sulfolipid from serum protein. This was followed by isolation of the sulfolipid on an anion-exchange column. Through this procedure, human serum was found to contain sulfotransferase activity. The serum enzyme was activated by Mn2+. Km values of the enzyme for GalCer and ''active sulfate'' were 4.6 .mu.M and 5.2 .mu.M, respectively. The enzyme activity was assayed in sera of cancer patients. The serum activity (mean .+-. SE, 0.27 .+-. 0.027 pmol .cntdot. .mu.l-1 .cntdot. h-1) in renal cell carcinoma patients, whose activity has been demonstrated to be elevated, was significantly (P < 0.005) increased compared to that of the normal control (mean .+-. SE, 0.18 .+-. 0.0014 pmol.cntdot..mu.l-1.cntdot.h-1) and of other urological tumors examined.