Population genetic analysis and sub-structuring in Babesia bovis
Population genetic analysis and sub-structuring in Babesia bovis
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DOI：
10.1016/j.molbiopara.2011.02.002
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发表时间：
2011-06-01
影响因子：
1.5
通讯作者：
Weir, William
中科院分区：
文献类型：
--
作者：
Simuunza, Martin;Bilgic, Huseyin;Weir, William
蜱传播的原生动物寄生虫，牛巴贝虫是引起牛巴贝虫病的原因之一，这是热带和亚热带国家牛的一种经济上重要的疾病。使用最近公布的寄生虫的基因组序列，我们开发了一个面板的8个迷你和微卫星标记，并使用这些调查的作用，遗传交换的人口结构和多样性的寄生虫使用分离株来自赞比亚和土耳其。这一群体遗传分析表明，遗传交换发生，有高水平的遗传多样性，地理子结构量化使用赖特的F指数。当将来自这两个国家的分离株作为一个群体处理时，观察到连锁不平衡，但当分别分析来自赞比亚的分离株时，观察到连锁平衡。土耳其分离物是亚结构的，包含两个遗传上不同的亚组，这两个亚组似乎都处于连锁平衡。赞比亚的研究结果表明，寄生虫种群的一个子集是巴贝斯虫病向西传播到该国以前无病的中部地区的原因。赞比亚的分离株有一个显着较高的基因型每个样本比那些从土耳其和年龄被发现是一个显着的预测多重感染。赞比亚和土耳其B族的高度多样性。牛的种群在针对该疾病的亚单位疫苗的开发和耐药性的传播方面具有意义。(C)2011 Elsevier B.V.保留所有权利。
The tick-borne protozoan parasite, Babesia bovis is one of the causes of bovine babesiosis, an economically important disease of cattle in tropical and sub-tropical countries. Using the recently published genome sequence of the parasite, we developed a panel of eight mini- and micro-satellite markers and used these to investigate the role of genetic exchange in the population structure and diversity of the parasite using isolates from Zambia and Turkey. This population genetic analysis showed that genetic exchange occurs and that there are high levels of genetic diversity, with geographical sub-structuring quantified using Wright's F Index. Linkage disequilibrium was observed when isolates from both countries were treated as one population, but when isolates from Zambia were analysed separately linkage equilibrium was observed. The Turkish isolates were sub-structured, containing two genetically distinct sub-groups, both of which appeared to be in linkage equilibrium. The results of the Zambian study suggest that a sub-set of the parasite population is responsible for the westward spread of babesiosis into the previously disease-free central region of the country. The Zambian isolates had a significantly higher number of genotypes per sample than those from Turkey and age was found to be a significant predictor of the multiplicity of infection. The high levels of diversity seen in the Zambian and Turkish B. bovis populations have implications in the development of subunit vaccines against the disease and the spread of drug resistance. (C) 2011 Elsevier B.V. All rights reserved.