A Peptide Derived from GAPDH Enhances Resistance to DNA Damage in Saccharomyces cerevisiae Cells
A Peptide Derived from GAPDH Enhances Resistance to DNA Damage in Saccharomyces cerevisiae Cells
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DOI：
10.1128/aem.02194-21
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发表时间：
2022-02-01
影响因子：
4.4
通讯作者：
Fu，Yu
中科院分区：
文献类型：
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作者：
Zhao，Xi;Lian，Xianqiang;Fu，Yu
社会行为不仅存在于高等生物中，也存在于为共同利益而相互作用的微生物中。在这里，我们报告说，芽殖酵母细胞相互作用后，暴露于DNA损伤的邻近细胞。用DNA损伤UV光照射的酵母细胞分泌信号肽，其可以增加暴露于DNA损伤应激的酵母细胞的存活。分泌的肽来源于甘油醛-3-磷酸脱氢酶（GAPDH），并且它诱导组中一部分酵母细胞的细胞死亡。这些数据表明，GAPDH衍生的肽在芽殖酵母的社会互动中起作用，以响应DNA损伤stress.IMPORTANCEMany研究表明，微生物，包括细菌和酵母，暴露于相同的应激源后，显示出对应激的耐受性增加。然而，其机制仍然未知。在这项研究中，我们报告了一个惊人的发现，即酿酒酵母细胞通过分泌一种有助于抵抗DNA损伤应激的肽来应对DNA损伤。虽然已经证明GAPDH除了在糖酵解中的作用外，在细胞中还具有许多关键功能，但这项研究表明GAPDH还参与了对DNA损伤应激的社会行为反应。这项研究打开了一个有趣的研究领域的大门，即微生物适应恶劣环境的社会活动。
Social behaviors do not exist only in higher organisms but are also present in microbes that interact for the common good. Here, we report that budding yeast cells interact with their neighboring cells after exposure to DNA damage. Yeast cells irradiated with DNA-damaging UV light secrete signal peptides that can increase the survival of yeast cells exposed to DNA-damaging stress. The secreted peptide is derived from glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and it induced cell death of a fraction of yeast cells in the group. The data suggest that the GAPDH-derived peptide serves in budding yeast’s social interaction in response to DNA-damaging stress.IMPORTANCEMany studies have shown that microorganisms, including bacteria and yeast, display increased tolerance to stress after exposure to the same stressor. However, the mechanism remains unknown. In this study, we report a striking finding that Saccharomyces cerevisiae cells respond to DNA damage by secreting a peptide that facilitates resistance to DNA-damaging stress. Although it has been shown that GAPDH possesses many key functions in cells aside from its well-established role in glycolysis, this study demonstrated that GAPDH is also involved in the social behaviors response to DNA-damaging stress. The study opens the gate to an interesting research field about microbial social activity for adaptation to a harsh environment.