Resistance Tests and Genetic Resources for Breeding Fusarium Root Rot Resistant Lettuce
Resistance Tests and Genetic Resources for Breeding Fusarium Root Rot Resistant Lettuce
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抗镰刀菌根腐病生菜选育的抗性试验及遗传资源
DOI：
10.2503/jjshs.73.105
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发表时间：
2004
影响因子：
--
通讯作者：
M. Tsukada
中科院分区：
文献类型：
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作者：
N. Tsuchiya;M. Fujinaga;H. Ogiso;Tomita Usui;M. Tsukada
采用莴苣品种对枯萎病根腐病的抗性测试方法，对日本和国外莴苣遗传资源的抗性进行了评价，以帮助我们的育种计划。将种子播种在由病原菌培养的麸皮和蛭石（麸皮：蛭石= 1:1.5,v/v）和商品园艺苗圃土（混合比例：1/10 ~ 1/40，w/w）混合而成的土壤中，其中每ml含有超过108个细胞的镰刀菌（Fusarium oxysporum f. sp. lactucae）。接种土壤后的种子塞盘在自然日长条件下，在15℃~ 35℃（平均25℃）的温室中保存30 ~ 40 d。1999 - 2000年，对日本产的78份基因型和国外产的77份以脆型生菜为主的品种进行了抗性试验。无论这些基因型的来源如何，不同基因型的疾病严重程度存在很大差异。在来自日本的品种中，许多“先锋”型品种对SB1-1（种族1）具有更强的抗性，“V莴苣”对SB1-1具有高度抗性，而“帝国”类型，“菊川102”和“暴雪”则高度敏感。然而，许多‘Vanguard’型品种，‘V Lettuce’对F9501（2号种族）高度敏感，而‘Kikugawa 102’和‘Blizzard’对F9501具有高度抗性。与从日本收集的遗传资源相比，从国外收集的材料显示出更多的抗性。‘Batavia Tezier’和‘Batavia Blonde de Paris’经测试对SB1- 1和F9501都具有抗性。
Using a method for testing a varietal resistance of lettuce to fusarium root rot, we evaluated the resistance of genetic resources of lettuce gathered from Japan and abroad as an aid to our breeding progrom. Seeds were sown in a mixture of nursery soil of pathogenic fungus-cultured bran and vermiculite (bran:vermiculite = 1:1.5, v/v) and commercial horticultural nursery soil (mixing ratio: 1/10∼1/40, w/w), that included more than 108 cells per ml of Fusarium oxysporum f. sp. lactucae. The seeded plug trays with the inoculated soil were kept for 30 to 40 days at 15 °C to 35 °C (mean of 25 °C ) in a glasshouse under natural daylength. At the end of incubation period, seeding were indexed for resistance to fusarium root rot. Resistance tests were conducted on 78 accessions of genotypes from Japan, and 77 accessions which were mainly crisp-type lettuce gathered from abroad between 1999 and 2000. Regardless of the sources of these genotypes, large differences were seen in disease severity among the genotypes. Among those from Japan, many of the 'Vanguard' type varieties possessed more resistance to isolate SB1-1 (Race 1) and 'V Lettuce' were highly resistance to isolate SB1-1, whereas 'Empire' types, 'Kikugawa 102' and 'Blizzard'were highly susceptible. However, many 'Vanguard' type varieties, 'V Lettuce' were highly susceptible to isolate F9501 (Race 2). while 'Kikugawa 102' and 'Blizzard' possessed highly resistance to isolate F9501. Compared to those genetic resources gathered from Japan, a larger number of materials from abroad showed resistance. 'Batavia Tezier' and 'Batavia Blonde de Paris' tested to be resistant to both SB1- 1 and F9501.