Alien introgression in the grasses Lolium perenne (perennial ryegrass) and Festuca pratensis (meadow fescue): the development of seven monosomic substitution lines and their molecular and cytological characterization
Alien introgression in the grasses Lolium perenne (perennial ryegrass) and Festuca pratensis (meadow fescue): the development of seven monosomic substitution lines and their molecular and cytological characterization
复制标题
DOI：
10.1093/aob/mcr083
复制
发表时间：
2011-06-01
期刊：
影响因子：
4.2
通讯作者：
King, Julie
中科院分区：
文献类型：
--
作者：
Harper, John;Armstead, Ian;King, Julie
背景和目的为了解决与粮食安全、环境变化和生物能源相关的问题，在作物植物的背景下，新植物表型的产生、鉴定和评价是至关重要的。实现这一目标的主要途径之一将是远缘杂交和渐渗育种。染色体和染色体片段在相关物种之间的转移（染色体工程或外来渐渗）也为确定目标性状的遗传控制提供了重要资源。然而，染色体工程的全部潜力的实现以前一直受到阻碍，无法识别和表征种间渗入accurately.Methods七个单体置换线已产生包括作为供体物种和多年生黑麦草作为受体的羊茅。这七条线中的每一条都有一个不同的L。perenne染色体被homoeoprotein F. Pratensis染色体（13 L. perenne + 1 F. Pratensis染色体）。利用分子标记和基因组原位杂交技术对F.在每一个单体置换中，Pratensis染色体渗入到一个特定的连锁群中。对各代换系减数分裂中期Ⅰ进行了细胞学观察。perenne和F.草地在中期我的观察发现，存在一个低水平的种间染色体易位这些species.Discussion之间的分离的7个单体置换线提供了一个资源解剖的遗传控制的重要性状和基因分离。L之间的相似之处。perenne F.草地系统和早熟禾亚科谷物如小麦、大麦、黑麦、燕麦和模式草二穗短柄草提供了在基因型和表型方面进行物种间比较的机会。
Background and Aims To address the issues associated with food security, environmental change and bioenergy in the context of crop plants, the production, identification and evaluation of novel plant phenotypes is fundamental. One of the major routes to this end will be wide hybridization and introgression breeding. The transfer of chromosomes and chromosome segments between related species (chromosome engineering or alien introgression) also provides an important resource for determining the genetic control of target traits. However, the realization of the full potential of chromosome engineering has previously been hampered by the inability to identify and characterize interspecific introgressions accurately.Methods Seven monosomic substitution lines have been generated comprising Festuca pratensis as the donor species and Lolium perenne as the recipient. Each of the seven lines has a different L. perenne chromosome replaced by the homoeologous F. pratensis chromosome (13 L. perenne + 1 F. pratensis chromosome). Molecular markers and genomic in situ hybridization (GISH) were used to assign the F. pratensis chromosomes introgressed in each of the monosomic substitutions to a specific linkage group. Cytological observations were also carried out on metaphase I of meiosis in each of the substitution lines.Results A significant level of synteny was found at the macro-level between L. perenne and F. pratensis. The observations at metaphase I revealed the presence of a low level of interspecific chromosomal translocations between these species.Discussion The isolation of the seven monosomic substitution lines provides a resource for dissecting the genetic control of important traits and for gene isolation. Parallels between the L. perenne/F. pratensis system and the Pooideae cereals such as wheat, barley, rye, oats and the model grass Brachypodium distachyon present opportunities for a comparison across the species in terms of genotype and phenotype.