The simian virus 40 core origin contains two separate sequence modules that support T-antigen double-hexamer assembly.
The simian virus 40 core origin contains two separate sequence modules that support T-antigen double-hexamer assembly.
复制标题
猿猴病毒40核心起源包含两个独立的序列模块，支持T抗原双六聚体组装。
DOI：
10.1128/jvi.74.18.8589-8600.2000
复制
发表时间：
2000
影响因子：
5.4
通讯作者：
Bullock,PA
中科院分区：
文献类型：
--
作者：
Sreekumar,KR;Prack,AE;Winters,DR;Barbaro,BA;Bullock,PA
Using subfragments of the simian virus 40 (SV40) core origin, we demonstrate that two alternative modules exist for the assembly of T-antigen (T-ag) double hexamers. Pentanucleotides 1 and 3 and the early palindrome (EP) constitute one assembly unit, while pentanucleotides 2 and 4 and the AT-rich region constitute a second, relatively weak, assembly unit. Related studies indicate that on the unit made up of pentanucleotide 1 and 3 and the EP assembly unit, the first hexamer forms on pentanucleotide 1 and that owing to additional protein-DNA and protein-protein interactions, the second hexamer is able to form on pentanucleotide 3. Oligomerization on the unit made up of pentanucleotide 2 and 4 and the AT-rich region is initiated by assembly of a hexamer on pentanucleotide 4; subsequent formation of the second hexamer takes place on pentanucleotide 2. Given that oligomerization on the SV40 origin is limited to double-hexamer formation, it is likely that only a single module is used for the initial assembly of T-ag double hexamers. Finally, we discuss the evidence that nucleotide hydrolysis is required for the remodeling events that result in the utilization of the second assembly unit.
