The effect of anticancer drugs on seven cell lines monitored by FTIR spectroscopy
The effect of anticancer drugs on seven cell lines monitored by FTIR spectroscopy
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DOI：
10.1039/c2an35116a
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发表时间：
2012-01-01
期刊：
影响因子：
4.2
通讯作者：
Goormaghtigh, Erik
中科院分区：
文献类型：
--
作者：
Derenne, Allison;Verdonck, Magali;Goormaghtigh, Erik
越来越需要代谢组学等系统方法来改进新药的开发。在本文中，我们提出了一种基于红外光谱的新策略，它可以探测样品的整体化学成分。研究了来自三种肿瘤类型的七种细胞株，并用四种经典抗癌药物作用于两类具有独特作用机制的药物。首先，分别考虑每个细胞系，并对这7个细胞系建立层次聚类。根据药物的作用机制，光谱对所有被测试的细胞系进行了分类。其次，同时研究了不同细胞系药物作用机制光谱指纹图谱的相似性。计算差谱(减去相应未处理细胞系的平均谱)，从而消除每个细胞系的特定贡献，并且只能比较药物诱导的差异。等级聚类显示了区分这两种作用模式的明显趋势，揭示了对具有相似机制的分子的非常相似的响应类型。现在已经有了96孔板的高通量系统，可以开发一种成熟的生物检测方法，为潜在的抗癌药物提供一个客观的分类器。
Systemic approaches such as metabolomics are increasingly needed to improve the development of novel drugs. In this paper, we suggest a new strategy based on infrared spectroscopy which probes the global chemical composition of a sample. Seven cell lines from three tumour types were investigated and exposed to four classical anticancer drugs belonging to two classes characterized by a unique mechanism. First, each cell line was considered separately and a hierarchical clustering was built for the seven cell lines. Spectra clustered according to the drug mechanism of action for all the cell lines tested. Second, the similarities among drug mechanism spectral fingerprints were investigated for all the cell lines simultaneously. Difference spectra (the mean spectrum of the corresponding untreated cell line was subtracted) were computed so that the particular contribution of every cell line was eliminated and only the drug-induced differences could be compared. The hierarchical clustering shows a clear tendency to distinguish the two modes of action, revealing a very similar type of response to molecules with a similar mechanism. High throughput systems with 96-well plates are now available and a well established bioassay could be developed in order to provide an objective classifier for potential anticancer drugs.