Comparison of N- and C-terminally endogenously GFP-tagged WEE-1.3 strains in C. elegans.
Comparison of N- and C-terminally endogenously GFP-tagged WEE-1.3 strains in C. elegans.
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比较C.优雅的
DOI：
10.17912/micropub.biology.000353
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发表时间：
2021-01-14
影响因子：
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通讯作者：
Allen AK
中科院分区：
文献类型：
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作者：
Fernando LM;Golliday K;Boateng R;Allen AK
我们在C.我们已经用GFP内源性标记了C末端。在该出版物中，我们证明了这种新菌株表现出与先前已出版的WEE-1.3抗体和N-末端内源性GFP标记的WEE-1.3菌株相同的表达定位模式。我们还首次表明，内源性标记WEE-1.3在任何一个终端不影响蠕虫的生殖功能。
We have generated a WEE-1.3 strain in C. elegans wherewe have endogenously tagged the C-terminus with GFP. In this publication we demonstrate that this new strain exhibits the same expression localization pattern as the WEE-1.3 antibody and N-terminally endogenously GFP-tagged WEE-1.3 strain that have been previously published. We also show for the first time that endogenously tagging WEE-1.3 at either termini does not affect the reproductive function of the worms.