IGF-1 Gene Transfer to Human Synovial MSCs Promotes Their Chondrogenic Differentiation Potential without Induction of the Hypertrophic Phenotype.
IGF-1 Gene Transfer to Human Synovial MSCs Promotes Their Chondrogenic Differentiation Potential without Induction of the Hypertrophic Phenotype.
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DOI：
10.1155/2017/5804147
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发表时间：
2017
影响因子：
4.3
通讯作者：
Nakamura N
中科院分区：
文献类型：
--
作者：
Ikeda Y;Sakaue M;Chijimatsu R;Hart DA;Otsubo H;Shimomura K;Madry H;Suzuki T;Yoshikawa H;Yamashita T;Nakamura N
基于间充质干细胞（MSC）的疗法是一种有前途的软骨治疗方法。然而，修复组织通常无法再生原始的透明样组织。在这项研究中，我们专注于提高胰岛素样生长因子-1 (IGF-1) 的表达水平，以改善修复组织的质量。用慢病毒载体将IGF-1基因导入人滑膜间充质干细胞中，并使用颗粒培养物检测软骨分化条件下间充质干细胞的基因表达水平和形态状态。来自 IGF-1-MSC 的颗粒尺寸明显大于对照组。 IGF-1-MSC 组的糖胺聚糖 (GAG) 丰度也显着更高。 IGF-1 诱导沉淀的组织学显示与透明软骨相似，但未表现出肥大软骨细胞表型的特征。 IGF-1 颗粒中 Col2A1 基因和蛋白质的表达水平显着高于对照颗粒，但 Col10、MMP-13、ALP 和 Osterix 的表达水平并不较高。因此，将 IGF-1 基因转移至人滑膜 MSC 导致软骨形成分化能力提高，而没有检测到肥大或成骨表型的诱导。
Mesenchymal stem cell- (MSC-) based therapy is a promising treatment for cartilage. However, repair tissue in general fails to regenerate an original hyaline-like tissue. In this study, we focused on increasing the expression levels for insulin-like growth factor-1 (IGF-1) to improve repair tissue quality. The IGF-1 gene was introduced into human synovial MSCs with a lentiviral vector and examined the levels of gene expression and morphological status of MSCs under chondrogenic differentiation condition using pellet cultures. The size of the pellets derived from IGF-1-MSCs were significantly larger than those of the control group. The abundance of glycosaminoglycan (GAG) was also significantly higher in the IGF-1-MSC group. The histology of the IGF-1-induced pellets demonstrated similarities to hyaline cartilage without exhibiting features of a hypertrophic chondrocyte phenotype. Expression levels for the Col2A1 gene and protein were significantly higher in the IGF-1 pellets than in the control pellets, but expression levels for Col10, MMP-13, ALP, and Osterix were not higher. Thus, IGF-1 gene transfer to human synovial MSCs led to an improved chondrogenic differentiation capacity without the detectable induction of a hypertrophic or osteogenic phenotype.
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影响因子：
7.5
作者：
Frisch J;Venkatesan JK;Rey-Rico A;Schmitt G;Madry H;Cucchiarini M
通讯作者：
Cucchiarini M
影响因子：
7.5
作者：
Marolt D;Knezevic M;Novakovic GV
通讯作者：
Novakovic GV
对医疗统计信息的自由使用的易于使用的软件“ EZR”的调查。
影响因子：
4.8
作者：
Kanda Y
通讯作者：
Kanda Y
影响因子：
3.1
作者：
Madry H;Kaul G;Zurakowski D;Vunjak-Novakovic G;Cucchiarini M
通讯作者：
Cucchiarini M
影响因子：
2.4
作者：
Huat TJ;Khan AA;Pati S;Mustafa Z;Abdullah JM;Jaafar H
通讯作者：
Jaafar H