Glucocorticoid receptor-cAMP response element-binding protein interaction and the response of the phosphoenolpyruvate carboxykinase gene to glucocorticoids.
Glucocorticoid receptor-cAMP response element-binding protein interaction and the response of the phosphoenolpyruvate carboxykinase gene to glucocorticoids.
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DOI：
10.1016/s0021-9258(18)53327-5
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发表时间：
1993-03
期刊：
影响因子：
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通讯作者：
E. Imai;J. Miner;J. Mitchell;Keith R. Yamamoto;D. Granner
中科院分区：
文献类型：
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作者：
E. Imai;J. Miner;J. Mitchell;Keith R. Yamamoto;D. Granner
磷酸烯醇式丙酮酸羧激酶（PEPCK）基因编码的限速酶在胚胎发育。糖皮质激素通过多组分调节复合物增强PEPCK基因表达。我们发现，对糖皮质激素的完全反应需要两个DNA片段：1）糖皮质激素反应单元（GRU），位于-400位点，包含两个辅助因子元件（AF 1和AF 2）和两个糖皮质激素受体结合位点（GR 1和GR 2），2）位于-90位点的基础启动子/环AMP反应元件（E/CRE），与转录因子CREB结合。GR和CREB之间在体外观察到蛋白质-蛋白质相互作用，这可能解释了E/CRE在PEPCK基因的糖皮质激素反应中的作用。
The phosphoenolpyruvate carboxykinase (PEPCK) gene encodes the rate-limiting enzyme in gluconeogenesis. Glucocorticoids enhance PEPCK gene expression through a multicomponent regulatory complex. We show that a full response to glucocorticoids requires two DNA segments: 1) a glucocorticoid response unit (GRU), centered at about position -400, which contains two accessory factor elements (AF1 and AF2) and two glucocorticoid receptor binding sites (GR1 and GR2), and 2) a basal promoter/cyclic AMP response element (E/CRE) at about position -90, which binds the transcription factor CREB. A protein-protein interaction was observed in vitro between GR and CREB that might account for the role of the E/CRE in the glucocorticoid response of the PEPCK gene.