Mechanical properties of radiation-sterilised human Bone-Tendon-Bone grafts preserved by different methods
Mechanical properties of radiation-sterilised human Bone-Tendon-Bone grafts preserved by different methods
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DOI：
10.1007/s10561-008-9112-1
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发表时间：
2009-08-01
影响因子：
1.5
通讯作者：
Zasacka, M.
中科院分区：
文献类型：
--
作者：
Kaminski, A.;Gut, G.;Zasacka, M.
髌腱自体和同种异体移植物常用于骨科手术中重建前交叉韧带（ACL）。自体移植物主要用于初次重建，而同种异体移植物用于翻修手术。为了避免传染病传播的风险，同种异体移植物应进行辐射消毒。由于辐射灭菌据说会降低肌腱的机械强度，因此建立同种异体移植物保存和灭菌方法以确保移植物的最佳质量及其安全性是很重要的。因此，本研究的目的是比较通过各种方法（深度新鲜冷冻、甘油化、冻干）保存并随后以0、25、50或100 kGy剂量进行辐射灭菌的人髌腱（切割用于ACL重建）的拉伸强度。骨-肌腱-骨移植物（BTB）由具有髌骨和胫骨附件的尸体人髌骨肌腱制备。通过深度冷冻、甘油化或冷冻干燥保存BTB移植物，随后用0（对照）、25、50或100 kGy的剂量进行辐射灭菌。使用Instron系统对所有样品进行机械失效拉伸试验，以估计其机械性能。所有冻干移植物在进行这些试验前均进行再水化。所获得的机械测试结果表明，深冻辐照移植物保留其初始的机械性能的程度，这并不排除其临床应用。
Patellar tendon auto- and allo-grafts are commonly used in orthopedic surgery for reconstruction of the anterior cruciate ligaments (ACL). Autografts are mainly used for primary reconstruction, while allografts are useful for revision surgery. To avoid the risk of infectious disease transmission allografts should be radiation-sterilised. As radiation-sterilisation supposedly decreases the mechanical strength of tendon it is important to establish methods of allograft preservation and sterilisation assuring the best quality of grafts and their safety at the same time. Therefore, the purpose of this study was to compare the tensile strength of human patellar tendon (cut out as for ACL reconstruction), preserved by various methods (deep fresh freezing, glycerolisation, lyophilisation) and subsequently radiation-sterilised with doses of 0, 25, 50 or 100 kGy. Bone-Tendon-Bone grafts (BTB) were prepared from cadaveric human patella tendons with both patellar and tibial attachments. BTB grafts were preserved by deep freezing, glycerolisation or lyophilisation and were subsequently radiation-sterilised with doses of 0 (control), 25, 50 or 100 kGy. All samples were subjected to mechanical failure tensile tests with the use of Instron system in order to estimate their mechanical properties. All lyophilised grafts were rehydrated before performing of those tests. Obtained mechanical tests results of examined grafts suggest that deep-frozen irradiated grafts retain their initial mechanical properties to an extent which does not exclude their clinical application.