Physical organization of subgroup B human adenovirus genomes
Physical organization of subgroup B human adenovirus genomes
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B亚型人类腺病毒基因组的物理组织
DOI：
10.1128/jvi.24.2.564-579.1977
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发表时间：
1977
影响因子：
5.4
通讯作者：
C. Tibbetts
中科院分区：
文献类型：
--
作者：
C. Tibbetts
九种细菌限制性核酸内切酶的切割位点被定位在 3 型腺病毒 (Ad3) 和 Ad7 的 DNA 中，它们是“弱致癌”B 亚组人类腺病毒的代表性血清型。在绘制的 94 个位点中，有 82 个位点是两种血清型所共有的，这与同一亚组成员之间 DNA 的总体高度同源性一致。在 Ad3 和 Ad7 DNA 中的位点中，只有不到 20% 对应于 Ad2 或 Ad5 DNA 中映射的限制性位点。后者血清型代表“非致癌”C亚型，与B亚型腺病毒的DNA仅有10%至20%的整体序列同源性。 Ad7 感染细胞的病毒 mRNA 的杂交图谱产生了复杂的物理图谱，与 Ad2 DNA 中早期和晚期基因簇的图谱几乎相同。因此，B亚型和C亚型的人类腺病毒的DNA序列在病毒进化过程中存在显着差异，但腺病毒基因组的复杂组织却严格保守。
Cleavage sites of nine bacterial restriction endonucleases were mapped in the DNA of adenovirus type 3 (Ad3) and Ad7, representative serotypes of the "weakly oncogenic" subgroup B human adenoviruses. Of 94 sites mapped, 82 were common to both serotypes, in accord with the high overall sequence homology of DNA among members of the same subgroups. Of the sites in Ad3 and Ad7 DNA, fewer than 20% corresponded to mapped restriction sites in the DNA of Ad2 or Ad5. The latter serotypes represent the "nononcogenic" subgroup C, having only 10 to 20% overall sequence homology with the DNA of subgroup B adenoviruses. Hybridization mapping of viral mRNA from Ad7-infected cells resulted in a complex physical map that was nearly identical to the map of early and late gene clusters in Ad2 DNA. Thus the DNA sequences of human adenoviruses of subgroups B and C have significantly diverged in the course of viral evolution, but the complex organization of the adenovirus genome has been rigidly conserved.