Comparison of microbial communities in four different composting processes as evaluated by denaturing gradient gel electrophoresis analysis
Comparison of microbial communities in four different composting processes as evaluated by denaturing gradient gel electrophoresis analysis
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DOI：
10.1046/j.1365-2672.2003.01949.x
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发表时间：
2003-01-01
影响因子：
4
通讯作者：
Takii, S
中科院分区：
文献类型：
--
作者：
Ishii, K;Takii, S
目的：我们旨在通过对四个全面堆肥过程中微生物群落的比较，系统地了解堆肥过程。方法与结果：采用变性梯度凝胶电泳法，结合理化参数测定，对4种不同规模堆肥过程中的微生物群落进行了分析。两个堆肥过程利用了污水污泥和两个利用了食物垃圾。结果表明，食物垃圾堆肥的溶解有机碳浓度高于污水污泥堆肥，微生物群落因堆肥基质的不同而不同。不同溶解有机碳浓度的堆肥过程中出现不同微生物的趋势与先前的一项研究一致，该研究表明，在实验室规模的食物垃圾堆肥过程中，微生物演代随着溶解有机碳的减少而发生。结论：堆肥过程中影响微生物群落的主要因素是溶出有机物的浓度。研究意义与影响：本研究除研究堆肥过程中涉及的微生物群落外，还首次采用分子方法研究堆肥机理。我们的研究结果可能有助于堆肥过程的设计和管理。
Aims: We aimed to systematically understand the composting processes by a comparison of microbial communities during four full-scale composting processes.Methods and Results: Microbial communities during the four different full-scale composting processes were analysed by denaturing gradient gel electrophoresis combined with measurement of physicochemical parameters. Two composting processes utilized sewage sludge and two utilized food-waste. Comparison of the four processes indicated that the concentration of dissolved organic carbon was higher in the food-waste-composting than in the sewage-sludge-composting processes, and microbial communities varied with composting substrate. The tendency for different microbes to appear in the composting process with different concentrations of dissolved organic carbon agreed with a previous study that showed that microbial succession occurred with a decrease in dissolved organic carbon in a laboratory-scale food-waste-composting process.Conclusions: Our results suggested that the main factor affecting microbial communities in the composting process is the concentration of dissolved organic materials.Significance and Impact of the Study: In addition to studying microbial communities involved in composting, this research is also the first to study composting mechanisms using molecular methods. The results of our studies may be helpful in the design and management of composting processes.