Qualitative and semiquantitative analysis of Lactobacillus species in the vaginas of healthy fertile and postmenopausal Chinese women
Qualitative and semiquantitative analysis of Lactobacillus species in the vaginas of healthy fertile and postmenopausal Chinese women
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中国健康生育期和绝经后女性阴道中乳酸菌的定性和半定量分析
DOI：
10.1099/jmm.0.038687-0
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发表时间：
2012-05-01
影响因子：
3
通讯作者：
Liao, Qinping
中科院分区：
文献类型：
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作者：
Zhang, Rui;Daroczy, Katalin;Liao, Qinping
本研究旨在调查中国健康女性阴道中乳酸菌的种类和数量。应用16S rRNA基因克隆文库和物种特异性聚合酶链式反应，结合测序和实时定量聚合酶链式反应，对92例正常生育妇女和22例绝经后健康妇女的阴道标本进行了分析。共检测到13种不同的乳杆菌。物种特异性聚合酶链式反应显示，3%的育龄妇女被一种乳杆菌定植，97%的育龄妇女被两种或两种以上乳杆菌定植。在绝经后妇女中，91%由一种乳杆菌定植，9%由两种乳杆菌定植。在育龄妇女中，伊氏乳杆菌(82.61%)、皱纹乳杆菌(70.65%)和加色乳杆菌(67.39%)的感染率高于简氏乳杆菌(40.22%)、嗜酸乳杆菌(39.13%)、短乳杆菌(23.91%)、植物乳杆菌(5.43%)、约翰逊乳杆菌(3.26%)、发酵乳杆菌(2.17%)、唾液乳杆菌(2.17%)、沙鼠乳杆菌(1.09%)，罗氏乳杆菌(1.09%)和副乳杆菌(1.09%)；未检出德氏乳杆菌。在绝经后妇女中，未检出发酵乳杆菌、鼠李氏乳杆菌、路氏乳杆菌和德氏乳杆菌，其他10种仅在少数样本中检出。根据克隆文库，这些物种的流行率与物种特异性聚合酶链式反应所显示的流行率不同。根据半定量分析，育龄妇女的总乳杆菌DNA浓度高于绝经后妇女。61%的育龄妇女主要由L.iners、35%和2%的L.asseri寄生。育龄妇女乳酸菌菌种间的关联性显著(P
The present study aimed to investigate the prevalence and quantity of Lactobacillus species in the vaginas of healthy Chinese women. Vaginal samples from 92 fertile and 22 postmenopausal healthy Chinese women were analysed using a 16S rRNA gene clone library and species-specific PCR followed by sequencing and real-time PCR. A total of 13 different Lactobacillus species were detected. Species-specific PCR showed that 3% of the fertile women were colonized by one species of Lactobacillus and 97% were colonized by two or more species. Among the postmenopausal women, 91% were colonized by one species of Lactobacillus and 9% were colonized by two species. In the fertile women, L. iners (82.61%), L. crispatus (70.65%) and L. gasseri (67.39%) were more prevalent than L. jensenii (40.22%), L. acidophilus (39.13%), L. brevis (23.91%), L. plantarum (5.43%), L. johnsonii (3.26%), L. fermentum (2.17%), L. salivarius (2.17%), L. rhamnosus (1.09%), L. reuteri (1.09%) and L. paracasei (1.09%); L. delbrueckii was not detected. In the postmenopausal women, L. fermentum, L. rhamnosus, L. reuteri and L. delbrueckii were not detected, and the other 10 species were detected in just a few samples. The prevalence of these species according to the clone library differed from the prevalence indicated by the species-specific PCR. According to the semiquantitative analysis, the total Lactobacillus DNA concentrations were higher in fertile women than in postmenopausal women. Sixty-one per cent of the fertile women were predominantly colonized by L. iners, 35% by L. crispatus, and 2% by L. gasseri. Associations between pairs of Lactobacillus species in fertile women were significant (P