Rapid and sensitive detection of alpha-fetoprotein by a magnetically promoted shake-free immunoassay employing fluorescent magnetic nanobeads
Rapid and sensitive detection of alpha-fetoprotein by a magnetically promoted shake-free immunoassay employing fluorescent magnetic nanobeads
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发表时间：
2014-09
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通讯作者：
K. Terada;Toshiyuki Tanaka;Naohiro Hanyu;T. Honda;H. Handa
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作者：
K. Terada;Toshiyuki Tanaka;Naohiro Hanyu;T. Honda;H. Handa
最近，报道了一种快速灵敏的荧光夹心免疫测定法，其具有使用荧光磁性纳米珠磁性促进免疫反应的适应性。该免疫测定包括振荡洗涤步骤以洗出未结合的荧光磁性纳米珠。在此，为了简化洗涤过程并提高洗涤效率，我们通过应用磁辅助来修改洗涤过程。改进的洗涤过程，这是磁辅助和无振荡，更容易执行，它增加了在检测的模型生物标志物甲胎蛋白（AFP）的信噪比。血清AFP的检出限和定量限分别为15.4pg/mL和23.5pg/mL。总测定时间在10分钟内。因此，建议的免疫分析与免振洗允许简单，快速，灵敏的抗原检测。由于这些有利的特征，改进的免疫测定很可能在即时护理装置的开发中找到应用。
Summary Recently, a rapid and sensitive fluorescent sandwich immunoassay was reported with an adaptation that magnetically promoted immunoreaction using fluorescent magnetic nanobeads. This immunoassay includes a shake-washing step to wash out the unbound fluorescent magnetic nanobeads. Here, to simplify the washing process and to enhance the wash efficiency, we modified the washing process by applying magnetic assistance. The modified washing process, which was magnetically assisted and shake-free, was easier to perform and it increased the signal-to-noise ratio in the detection of a model biomarker alpha-fetoprotein (AFP). The limit of detection and the limit of quantification of serum AFP were 15.4 pg/mL and 23.5 pg/mL, respectively. The total assay time was within 10 min. Consequently, the proposed immunoassay with shake-free washing allowed simple, rapid, and sensitive detection of the antigen. Because of these advantageous features, the modified immunoassay is highly likely to find application in the development of point-of-care devices.