A TRANSGENIC IMMUNOGLOBULIN-MU GENE PREVENTS REARRANGEMENT OF ENDOGENOUS GENES
A TRANSGENIC IMMUNOGLOBULIN-MU GENE PREVENTS REARRANGEMENT OF ENDOGENOUS GENES
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DOI：
10.1016/s0092-8674(85)80107-0
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
BALTIMORE, D
中科院分区：
文献类型：
--
作者：
WEAVER, D;COSTANTINI, F;BALTIMORE, D
研究了含有重排免疫球蛋白H链基因的转基因小鼠对内源性免疫球蛋白基因DNA重排的影响。Abelson小鼠白血病病毒(A-MuLV)细胞系是从转基因小鼠和正常胎鼠的前B细胞中分离得到的。显微注射。MU。在每个转基因A-MuLV细胞系中都合成了基因RNA和α-H链蛋白。只有10%的正常小鼠A-MuLV转化子合成了MU。蛋白。在40%的转基因株系中观察到胚系JH等位基因，表明对内源免疫球蛋白DNA重排的阻断发生在H链DNA连接的第一步。在正常小鼠A-MuLV株中，所有等位基因均发生重排。在10%来源于增殖B细胞的转基因杂交瘤中也检测到了胚系JH等位基因。这一结果支持了一个通过成功重排的乘积来主动防止重排的模型。基因。
Transgenic mice containing a microinjected rearranged Ig .mu.H chain gene were examined for the effects on DNA rearrangement of the endogenous Ig genes. Abelson murine leukemia virus (A-MuLV) cell lines were isolated from pre-B cells of transgenic mice and of normal littermates. Microinjected .mu. gene RNA and a .mu.H chain protein were synthesized in every transgenic A-MuLV cell line. Only 10% of normal mouse A-MuLV transformants synthesized .mu. protein. A germ-line JH allele was observed in 40% of the transgenic lines, demonstrating that the block to endogenous Ig DNA rearrangement occurred at the 1st step of H chain DNA joining. All alleles were rearranged in normal mouse A-MuLV lines. Germline JH alleles were also detected in 10% of the transgenic hybridomas derived from proliferating B cells. The results support a model of active prevention of rearrangement by the product of successfully rearranged .mu. genes.