Molecular sequence analysis of prokaryotic diversity in the middle and outer sections of the Portuguese estuary Ria de Aveiro
Molecular sequence analysis of prokaryotic diversity in the middle and outer sections of the Portuguese estuary Ria de Aveiro
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DOI：
10.1016/j.femsec.2004.04.003
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发表时间：
2004-08-01
影响因子：
4.2
通讯作者：
Correia, A
中科院分区：
文献类型：
--
作者：
Henriques, IS;Almeida, A;Correia, A
16S rDNA文库的建设是为了研究葡萄牙Ria de Aveiro河口系统中自由生活的细菌群落的结构。样本于2002年4月从代表海洋（N 1站）和咸淡水（16站）的两个地点收集。克隆的RFLP图谱和序列分析表明，两个文库均以α -变形菌属为主，其次是γ -变形菌属和β -原菌属。与delta-proteobacteria、Verrucomicrobia、Bacleroidcles和actinobacteria相关的克隆不存在于16站，与厚壁菌门相关的序列只存在于n1站。检索到的序列仅包含在少数主要的细菌分裂中，并且通常与核苷酸数据库中存储的序列具有高度的同源性，并且从不同地理区域的水生环境中恢复。两个地点之间的差异可能反映了对不同环境条件的适应，特别是盐度。原核生物多样性模式与以往研究的其他沿海和河口环境相似。(C) 2004年欧洲微生物学会联合会。Elsevier B.V.版权所有。
Construction of 16S rDNA libraries was undertaken to examine the structure of free-living bacterial communities in the estuarine system Ria de Aveiro, Portugal. Samples were collected in April 2002, from two sites representing marine (station N 1) and brackish (station 16) water zones. Clones were characterized by RFLP patterns and sequence analysis of representative clones revealed that both libraries were clearly dominated by alpha-proteobacteria, followed by gamma-proteobacteria and beta-proieohaeteria. Clones affiliated with the delta-proteobacteria, Verrucomicrobia, Bacleroidcles and Actinobacieria were exclusive of station 16 and sequences related to the Firmicutes were only found in station N 1. Sequences retrieved are included in only a few major bacterial divisions and in general, shared a high degree of homology with sequences deposited in nucleotide databases, and recovered from aquatic environments of diverse geographic regions. Differences between the two sites may reflect adaptation to different environmental conditions, especially salinity. The pattern of prokaryotic diversity is comparable to other coastal and estuarine environments previously studied. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.