Perception of environmental signal by a marine diatom
Perception of environmental signal by a marine diatom
复制标题
DOI：
10.1126/science.288.5475.2363
复制
发表时间：
2000-06-30
期刊：
影响因子：
56.9
通讯作者：
Bowler, C
中科院分区：
文献类型：
--
作者：
Falciatore, A;d'Alcalà, MR;Bowler, C
硅藻是海洋生态系统的关键组成部分，对于二氧化硅的生物地球化学循环极其重要，并且是全球固定碳的贡献者。然而，诸如硅藻能够感知环境中的什么、它们如何响应外部信号以及哪些因素控制它们的生活策略等基本问题的答案在很大程度上是未知的。我们生成了含有钙敏感发光蛋白水母发光蛋白的转基因硅藻细胞，以确定钙稳态的变化是否用于响应相关的环境刺激。我们的研究结果揭示了用于检测和响应流体运动（剪切应力）、渗透应力和铁（控制海洋中硅藻丰度的关键营养素）的传感系统。
Diatoms are a key component of marine ecosystems and are extremely important for the biogeochemical cycling of silica and as contributors to global fixed carbon. However, the answers to fundamental questions such as what diatoms can sense in their environment, how they respond to external signals, and what factors control their Life strategies are largely unknown. We generated transgenic diatom cells containing the calcium-sensitive photoprotein aequorin to determine whether changes in calcium homeostasis are used to respond to relevant environmental stimuli. Our results reveal sensing systems for detecting and responding to fluid motion (shear stress), osmotic stress, and iron, a key nutrient that controls diatom abundance in the ocean.