An effect of hyposmolarity on insulin release in vitro.
An effect of hyposmolarity on insulin release in vitro.
复制标题
低渗透压对体外胰岛素释放的影响。
DOI：
10.1152/ajplegacy.1975.228.3.706
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
A. Renold
中科院分区：
文献类型：
--
作者：
W. Blackard;M. Kikuchi;A. Rabinovitch;A. Renold
培养基渗透压突然降低20mosm，引起了分散的短期胰岛素分泌反应，来自周围的切碎胰腺或分离的胰岛。胰岛素反应比葡萄糖或甲苯丁胺诱导的反应发生得早。通常的胰岛素分泌调节剂都不能显著改变这种分泌反应。糖酵解抑制剂、肾上腺素能激动剂和阻滞剂、胆碱能阻滞剂、有丝分裂纺锤体抑制剂和影响钠泵活性的药物未能改变低盐诱导的胰岛素分泌。操作灌注介质钙浓度是唯一的程序测试影响分泌反应。介质钙浓度的扰动增加了组织-介质钙梯度，增强了低摩尔诱导的胰岛素反应，而那些降低了组织-介质钙梯度的扰动则大大抑制了胰岛素反应。胰岛素对沐浴液渗透压降低的反应的确切原因尚不清楚；然而，这种刺激并不是胰岛素所特有的，因为低摩尔刺激也会引起胰高血糖素和淀粉酶的增加。
An abrupt reduction of medium osmolarity by as little as 20 mosM evoked a discrete short-lived insulin secretory response from perifused chopped pancreas or isolated islets. The insulin response occurred earlier than that induced by either glucose or tolbutamide. None of the usual modifiers of insulin secretion significantly altered this secretory response. Glycolytic inhibitors, adrenergic agonists and blocking agents, cholinergic blocking agents, mitotic spindle inhibitors, and agents influencing sodium pump activity failed to alter hyposmolar-induced insulin secretion. Manipulation of the perifusion medium calcium concentration was the only procedure tested that influenced the secretory response. Perturbations of medium calcium concentration that increased the tissue-to-medium calcium gradient augmented the hyposmolar-induced insulin response and those that decreased tissue-to-medium calcium gradient greatly inhibited the response. The precise cause of the insulin response to a decrease in bathing fluid osmolarity remains undefined; however, the stimulus is not specific for insulin because increases in glucagon and amylase were also elicited by the hyposmolar stimulus.