Genetic diversity of Moestrupia oblonga (Dinophyceae) from coastal areas of Okinawa Island, Japan.
Genetic diversity of Moestrupia oblonga (Dinophyceae) from coastal areas of Okinawa Island, Japan.
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日本冲绳岛沿海地区 Moestrupia oblonga（甲藻纲）的遗传多样性。
DOI：
10.1007/s12526-015-0351-7
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发表时间：
2015
影响因子：
1.6
通讯作者：
Shoichiro Suda
中科院分区：
文献类型：
--
作者：
Danang Ambar Prabowo;M.M.R. Shah;Takeo Horiguchi;Shoichiro Suda
研究了日本冲绳沿岸一种形态学鉴定为Moestrupia oblonga的底栖甲藻的遗传多样性。所有品系的形态相似，如无装甲细胞的米粒状，扣带位移约三分之一的体长，一个独特的腹凸缘，和一个明显的中央核，而核位于次锥，符合的一般形态M。oblonga（Larsen和Patterson）汉森和Daugbjerg，来自西班牙加那利岛特内里费。基于LSU rDNA D1-D3和SSU-ITS1 - 5. 8S-ITS2-LSU rDNA序列的分子系统发育分析表明，所有菌株都是单系的，但分为3个遗传上不同的分支，即分支A、B和C。对于所有分子标记，除了分化体B和分化体C的ITS1 - 5.8S rDNA-ITS2区域外，各分化体中的分化体内序列差异小于0.3%，所述分化体B和分化体C的ITS1 - 5.8S rDNA-ITS2区域在其成员之间分别显示0.7%和1.75%的序列差异。在SSU rDNA序列中，进化枝间的平均分化率为1.2 - 3.7%，而ITS和LSU rDNA序列中的核苷酸分化率分别为18 - 26%和6.3 - 9.7%。尽管冲绳的三个分支和M. oblonga（JF272764）在LSU rDNA D1-D3同源性方面显示出密切的亲和力，它们在碱基对方面与该属的唯一代表性有约7.5 - 12%的差异。此外，这些分支的形成与基于细胞大小的形态分化有些一致，表明这些分支可能代表了该属中的三个假隐种，与mM分离。来自西班牙特内里费岛的椭圆形。这项研究表明，属Moestrupiais比以前的报告提出的复杂得多。
The genetic diversity of marine benthic dinoflagellate strains morphologically identified asMoestrupia oblongafrom the coastal areas of Okinawa Island, Japan, were investigated. All strains shared similar morphologies, such as unarmored cells with a rice grain-like shape, the cingulum displacing about one third of the body length, a distinctive ventral flange, and a conspicuous central pyrenoid, while the nucleus was positioned in the hypocone, conforming to the general morphology ofM. oblonga(Larsen and Patterson) Hansen and Daugbjerg from Tenerife, Canary Island, Spain. Molecular phylogenetic analyses based on LSU rDNA D1-D3 and the concatenated dataset of SSU-ITS1-5.8S-ITS2-LSU rDNA sequences indicated that all strains are monophyletic, but separated into three genetically distinct clades; namely, Clade A, B, and C. Intraclade sequence divergence in the respective clades was less than 0.3 % for all molecular markers, excluding the ITS1-5.8S rDNA-ITS2 region of Clade B and Clade C that showed sequence divergence of 0.7 % and 1.75 % among its members, respectively. The mean interclade divergences between clades were about 1.2–3.7 % in SSU rDNA, but reached 18–26 % and 6.3–9.7 % for nucleotides in ITS and LSU rDNA sequences, respectively. Despite the link between the three Okinawan clades andM. oblonga(JF272764) from Spain showing close affinity in LSU rDNA D1-D3 phylogeny, they diverged about 7.5–12 % regarding base pairs from this sole representative of the genus. Moreover, the formation of these clades was somewhat consistent with morphological differentiation based on cell sizes, indicating that these clades may represent three pseudo-cryptic species in the genus, discreet fromM. oblongafrom Tenerife, Spain. This study reveals that the genusMoestrupiais much more complex than previous reports have suggested.