Spectroscopic study of bio-functionalized nanodiamonds
Spectroscopic study of bio-functionalized nanodiamonds
复制标题
DOI：
10.1016/j.diamond.2005.11.019
复制
发表时间：
2006-04-01
影响因子：
4.1
通讯作者：
Cheng, C. -L.
中科院分区：
文献类型：
--
作者：
Chung, P. -H.;Perevedentseva, E.;Cheng, C. -L.
表面附着有生物分子的纳米材料可用作细胞和组织分析中的生物探针。这些纳米颗粒与生物分子的结合是生物探针开发的关键步骤之一。在这项工作中，纳米尺寸的金刚石（5和100 nm）的功能化和共轭蛋白质溶菌酶通过物理吸附。利用傅里叶变换红外光谱（FTIR）研究了纳米金刚石表面官能团的形成过程。用紫外/维斯和红外光谱分析了纳米金刚石与生物分子的结合以及纳米金刚石与生物分子的相互作用。(c)2005 Elsevier B. V.保留所有权利。
Nano-scale materials with attached biomolecules on the surface can be used as bioprobes in cell and tissue analysis. The conjugation of these nanoparticles with biomolecules is one of the key steps in the development of bioprobes. In this work nanometer-sized diamonds (5 and 100 nm) are functionalized and conjugated with protein lysozyme via physical adsorption. The process of creating functional groups on the nanodiamond surface is studied using Fourier Transform Infrared-spectroscopy (FTIR). The conjugation of nanodiamonds with biontolecules and nanodiamond-biomolecule interaction are analyzed with UV/VIS and FTIR spectroscopy. (c) 2005 Elsevier B.V. All rights reserved.