High-throughput screening of cocaine, adulterants, and diluents in seized samples using capillary electrophoresis with capacitively coupled contactless conductivity detection
High-throughput screening of cocaine, adulterants, and diluents in seized samples using capillary electrophoresis with capacitively coupled contactless conductivity detection
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DOI：
10.1016/j.talanta.2020.120987
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发表时间：
2020-09-01
期刊：
影响因子：
6.1
通讯作者：
Silva, Sidnei G.
中科院分区：
文献类型：
--
作者：
Barreto, Diandra N.;Ribeiro, Michelle M. A. C.;Silva, Sidnei G.
可卡因是世界上使用最频繁的非法药物之一。因此，开发简便、快速的可卡因及常见的掺杂物、稀释剂和杂质的检测方法在法医学调查中极为重要。本研究首次描述了一种基于毛细管电泳电容耦合非接触式电导检测（CE-（CD）-D-4）的快速（2.5 min）和同时定量可卡因，左旋咪唑，利多卡因，碳酸盐，硼酸盐，氯化物，硝酸盐，亚硝酸盐和硫酸盐的方法。实验中，以10.0 mmol L ~（-1）TAPS、12 mmol L ~（-1）NaOH和0.2 mmol L ~（-1）CTAB组成的缓冲溶液（pH = 8.8）为背景电解质，阴离子以共电渗方式分离，阳离子以反电渗方式分离。开发的CE方法证明了一些有趣的分析特性，如：（i）一个简单的样品预处理步骤（仅在水中稀释和过滤），（ii）高通量筛选（24注射h（-1）），（iii）适当的回收率值（72和118%之间），（iv）所有分析物的日间精密度小于7%。该程序已成功应用于分析2018年期间由米纳斯吉拉斯民警综合法医站（PPI）（巴西米纳斯吉拉斯州乌贝拉巴）收集的缉获可卡因样本。
Cocaine is one of the most frequently used illicit drug in the world. Therefore, the development of simple and fast methods for the detection of cocaine and common adulterants, diluents and impurities are extremely important in forensic investigations. The present study describes, for the first time, a method based on capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-(CD)-D-4) for the rapid (2.5 min) and simultaneous quantification of cocaine, levamisole, lidocaine, carbonate, borate, chloride, nitrate, nitrite and sulphate. In the experiment, anions were separated in co-electroosmotic mode and cations in counter-electroosmotic mode employing a buffer solution composed by 10.0 mmol L-1 TAPS, 12 mmol L-1 NaOH and 0.2 mmol L-1 CTAB as the background electrolyte (pH = 8.8). The developed CE method demonstrated some interesting analytical characteristics such as: (i) a simple sample pretreatment step (only dilution in water and filtering), (ii) high-throughput screening (24 injections h(-1)), (iii) proper recovery values (between 72 and 118%), and (iv) an inter-day precision of less than 7% for all analytes. The procedure was successfully applied in the analysis of seized cocaine samples collected by the Integrated Forensics Post (PPI) of the Minas Gerais Civil Police (Uberaba, Minas Gerais State, Brazil), during the year of 2018.