Ethanol production from dilute acid hydrolysate of rice hulls using genetically engineered Escherichia coli

Ethanol production from dilute acid hydrolysate of rice hulls using genetically engineered Escherichia coli
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DOI:
10.1023/a:1005490501736
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发表时间:
1998-10-01
影响因子:
2.7
通讯作者:
Ingram, LO
Ingram, LO
中科院分区:
工程技术4区
文献类型:
--
作者:
Moniruzzaman, M;Ingram, LO

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大约 30% 的稻壳(代表了几乎没有商业价值的丰富残留物)用 0.4 M H2SO4 酸溶解,生产出含有超过 100 g 单糖/升的糖浆。 Ca(OH)(2) 可减轻水解过程中产生的毒素。处理过的水解产物加上额外的营养物质与大肠杆菌 KO11 一起发酵,在 72 小时内产生超过 46 g 乙醇/L(理论产量的 92%)。
Approximately 30% of rice hulls, which represent an abundant residue with little commercial value, was solubilized with 0.4 M H2SO4 acid to produce a syrup containing over 100 g monomer sugar/l. Toxins generated during hydrolysis were mitigated with Ca(OH)(2). Treated hydrolysate plus additional nutrients was fermented with Escherichia coli KO11 to produce over 46 g ethanol/L in 72 h (92% of theoretical yield).