Age-dependent activation of PKC isoforms by angiotensin II in the proximal nephron
Age-dependent activation of PKC isoforms by angiotensin II in the proximal nephron
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DOI：
10.1152/ajpregu.2001.281.3.r861
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发表时间：
2001-09-01
影响因子：
2.8
通讯作者：
Garvin, JL
中科院分区：
文献类型：
--
作者：
Boesch, DM;Garvin, JL
ANG II增加幼鼠近端小管的液体吸收比成年大鼠多。ANG II增加近端肾元的液体吸收，部分是通过激活蛋白激酶C （PKC）。然而，尚不清楚随着动物的成熟，ANG ii诱导的PKC级联刺激的年龄相关变化有何不同。我们假设，随着大鼠成熟，近端肾元对ANG II的反应降低是由于PKC的数量和激活的减少，而不是ANG II受体的数量或亲和力的减少。由于PKC在激活后会从细胞质转移到膜上，我们首先测量了幼鼠（26 +/-1日龄）和成年大鼠（54 +/-1日龄）暴露于载体或10(-10)M ANG II的近端小管匀浆的可溶性和颗粒部分PKC的活性。ANG II在幼年大鼠均质液中同样程度地增加PKC活性（从0.119 +/-0.017增加到0.146 +/-0.015 U/mg蛋白）
ANG II increases fluid absorption in proximal tubules from young rats more than those from adult rats. ANG II increases fluid absorption in the proximal nephron, in part, via activation of protein kinase C (PKC). However, it is unclear how age-related changes in ANG II-induced stimulation of the PKC cascade differ as an animal matures. We hypothesized that the response of the proximal nephron to ANG II decreases as rats mature due to a reduction in the amount and activation of PKC rather than a decrease in the number or affinity of ANG II receptors. Because PKC translocates from the cytosol to the membrane when activated, we first measured PKC activity in the soluble and particulate fractions of proximal tubule homogenates exposed to vehicle or 10(-10) M ANG II from young (26 +/-1 days old) and adult rats (54 +/-1 days old). ANG II increased PKC activity to the same extent in homogenates from young rats (from 0.119 +/-0.017 to 0.146 +/-0.015 U/mg protein) (P