Continuous biocatalytic recovery of neodymium and europium
Continuous biocatalytic recovery of neodymium and europium
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DOI：
10.1039/c4ra14892d
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发表时间：
2015-01
期刊：
影响因子：
3.9
通讯作者：
A. Murray;Sarah Singh;Dimitrios A. Vavlekas;M. R. Tolley;L. Macaskie
中科院分区：
文献类型：
--
作者：
A. Murray;Sarah Singh;Dimitrios A. Vavlekas;M. R. Tolley;L. Macaskie
利用沙雷氏菌的分批培养细胞和连续生长的生物膜从溶液中回收稀土元素镧和钕。选择性获得La（III）超过Th（IV）使用聚丙烯酰胺凝胶固定化细胞的挑战，在快速流速柱，利用不同的溶液化学和行为的稀土（III）和Th（IV）。生物膜生长的细胞有10倍高的活性介导的磷酸酶，促进金属沉积作为相应的金属磷酸盐，反映为相应的增强水平的去除Nd（III）（作为NdPO 4）在流通柱利用生物膜上的网状泡沫。生物膜在去除Nd（III）中保持活性>1年，以指数方式失去活性，半衰期为3个月。使用两种独立的生物膜制备物，在pH 5.5下通过3个月的生物膜得到50%的Nd（III）去除率（FA 1/2）的流速为272和275 mL h-1，相当于新鲜生物膜的FA 1/2为34柱体积/h。Nd（III）的去除在pH值下降至3.5时持续约10分钟。当返回至pH 5.5时，20%的柱活性损失。在常见的稀土浸出剂硫酸铵（100 mM）的存在下，发生了类似的结果，但这并不影响沙雷氏菌属的能力。鉴于未来的生物制造的Nd（III）-催化剂的潜力，沉积的材料被确定为NdPO 4通过X-射线粉末衍射与14.5 nm的纳米颗粒尺寸，无论生物膜的年龄。
Batch-grown cells and a continuously-grown biofilm of a Serratia sp. were utilized to recover the rare earth elements (REEs) lanthanum and neodymium from solution. Selectivity was obtained for La(III) over Th(IV) using columns of polyacrylamide gel-immobilized cells challenged at a rapid flow rate, exploiting the different solution chemistries and behaviors of REEs(III) and Th(IV). Biofilm-grown cells had a ten-fold higher activity of mediating phosphatase, which promotes metal deposition as the corresponding metal phosphate, reflected as a correspondingly enhanced level of removal of Nd(III) (as NdPO4) in flow-through columns utilizing the biofilm on reticulated foam. The biofilms retained activity in the removal of Nd(III) for >1 year, losing activity exponentially with a half life of 3 months. The flow rate giving 50% removal (FA1/2) of Nd(III) by 3 month old biofilms at pH 5.5 was 272 and 275 mL h−1 using two independent biofilm preparations, equivalent to a FA1/2 of 34 column volumes per h for fresh biofilms. The removal of Nd(III) was sustained at pH values down to 3.5 with approx. 20% of the column activity lost upon return to pH 5.5. A similar result occurred in the presence of the common REE leaching agent ammonium sulfate (100 mM), but this did not affect the ability of Serratia sp. to recover REEs. With a view to the potential for future biomanufacturing of Nd(III)-catalysts, the deposited material was identified as NdPO4 by X-ray powder diffraction with a nanoparticle size of 14.5 nm, irrespective of the biofilm age.