Genetic diversity of relictual and endangered plant Abies ziyuanensis (Pinaceae) revealed by AFLP and SSR markers
Genetic diversity of relictual and endangered plant Abies ziyuanensis (Pinaceae) revealed by AFLP and SSR markers
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AFLP和SSR标记揭示了孑遗濒危植物紫苑冷杉（松科）的遗传多样性
DOI：
10.1007/s10709-007-9178-x
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发表时间：
2008-05-01
期刊：
影响因子：
1.5
通讯作者：
Zhong, Yang
中科院分区：
文献类型：
--
作者：
Tang, Shaoqing;Dai, Wenjuan;Zhong, Yang
资源冷杉（Abies ziyuanensis）是我国南方特有的一种高度濒危的冷杉。与分布于寒冷地区的冷杉不同，冷杉属的冷杉属的冷杉。紫苑分布于亚热带山区的几个孤立的岛屿状地点。本研究利用显性扩增片段长度多态性（AFLP）和共显性简单重复序列（SSR）标记对黑木耳的遗传结构进行了分析。紫苑。7个种群，包括139个个体，在其整个分布进行了抽样。A.紫苑特有种的遗传多样性水平较低，平均每个居群的遗传多样性（He）分别为0.136（AFLP）和0.337（SSR），低于其它已报道的特有种。在7个群体中，AFLP标记的群体分化系数Gst = 0.482，SSR标记的群体分化系数Fst = 0.250。AMOVA也检测到两种标记类型在居群间（Phi st（AFLP）= 0.550和Phi st（SSR）= 0.289）和地区间（Phi ct（AFLP）= 0.139和Phi ct（SSR）= 0.135）的显著分化。两种正在进行的进化力量（例如，由小群体大小引起的遗传漂变）和历史事件（例如，在第四纪冰期旋回期间和之后的种群收缩和破碎化）可能对A.紫苑。
Abies ziyuanensis is a highly endangered fir species endemic to South China. Unlike other Abies species that are distributed in areas with cold climates, A. ziyuanensis is restricted to several isolated island-like localities at subtropical mountains. In this study, we used dominant amplified fragment length polymorphism (AFLP) and co-dominant simple sequence repeats (SSR) markers to infer the genetic structure of A. ziyuanensis. Seven populations consisting of 139 individuals were sampled across their whole distribution. A. ziyuanenesis has a relatively low level of genetic variation, with a mean genetic diversity per population (He) of 0.136 (AFLP) and 0.337 (SSR), which is lower than that of other reported endemic species based on the same kind of marker. We observed high population differentiation, with Gst = 0.482 (AFLP) and Fst = 0.250 (SSR), among the seven populations. AMOVA also detected significant differentiation among populations (Phi st (AFLP) = 0.550 and Phi st (SSR) = 0.289) and among regions (Phi ct (AFLP) = 0.139 and Phi ct (SSR) = 0.135) in both marker types. Both ongoing evolutionary forces (e.g., genetic drift resulting from small population size) and historical events (e.g., population contraction and fragmentation during and after the Quaternary glacial cycles) may have contributed to the genetic structure in A. ziyuanensis.