The Production of Plasma Activated Water in Controlled Ambient Gases and its Impact on Cancer Cell Viability
The Production of Plasma Activated Water in Controlled Ambient Gases and its Impact on Cancer Cell Viability
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在受控环境气体中等离子体活化水的产生及其对癌细胞活力的影响
DOI：
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发表时间：
2021
影响因子：
3.6
通讯作者：
E. Vasar
中科院分区：
文献类型：
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作者：
S. Raud;J. Raud;I. Jõgi;Carl‐Thomas Piller;T. Plank;R. Talviste;T. Teesalu;E. Vasar
本研究探讨了血浆产生的活性氧(ROS)和氮(RNS)物种对癌细胞活力的影响。通过在受控环境气体(空气、O2或氮气)中使用大气压Ar等离子体射流，在去离子水中产生活性物种，从而允许产生仅含有ROS(例如，O3、H2O2)或同时含有ROS和RNS(例如，H2O2、NO2-、NO3-)的等离子体活化水。在所有环境气体中都会产生相当数量的过氧化氢，其中氮气中过氧化氢的生成率最高，氧气中过氧化氢的生成率最低。后者与H_2O_2前驱体OH相连，在O2环境中有效猝灭。在环境空气和氮气中进行短时间(<； 5分钟)等离子体处理时，会产生少量的亚硝酸根。在氮气环境中，NO3-的生成量最高。只有在氧气环境下才能检测到臭氧。使用两种癌细胞系：4T1(乳腺癌)和PPC-1(前列腺癌)进行细胞活性研究。琥珀酸脱氢酶活性的比色测定结果表明，所研究的细胞系对等离子体激活的培养液具有相似的敏感性。在氧气环境中产生的介质的影响取决于过氧化氢的含量。在氮气环境中产生的等离子体激活介质中等量的过氧化氢导致的生存能力几乎是在氧气环境气体中的两倍。推测这是由于NH3对细胞增殖的促进作用。
The present study investigated the effect of plasma-produced reactive oxygen (ROS) and nitrogen (RNS) species on cancer cell viability. Reactive species were generated in deionized water by using an atmospheric pressure Ar plasma jet within the controlled ambient gases (air, O2 or N2), which allowed the production of plasma-activated water containing only ROS (e.g. O3, H2O2) or both ROS and RNS (e.g. H2O2, NO2–, NO3–). A considerable amount of H2O2 was produced in all ambient gases, and its generation rate was highest in N2 and lowest in O2. The latter was connected with a H2O2 precursor, OH, efficient quenching in O2 ambient gas. Small quantities of NO2– were generated during short (< 5 min) plasma treatments in ambient air and N2. The highest amount of NO3– was produced in N2 ambient gas. Ozone was detected only in the case of O2 environment. Cell viability studies were carried out by utilizing two cancer cell lines: 4T1 (breast cancer) and PPC-1 (prostate cancer). The results of the colorimetric succinate dehydrogenase activity assay showed that the studied cell lines had a similar sensitivity to the plasma activated medium. The impact of medium produced in the O2 ambient environment was determined by H2O2 content. The equivalent amount of H2O2 in the plasma activated medium produced in the N2 ambient environment caused an almost two-fold higher viability than in the case of the O2 ambient gas. It is proposed that this was due to the cellular proliferation enhancing effect of NH3.