Synthesis of Selenomethylene-locked Nucleic Acid (SeLNA)-modified Oligonucleotides by Polymerases
Synthesis of Selenomethylene-locked Nucleic Acid (SeLNA)-modified Oligonucleotides by Polymerases
复制标题
聚合酶合成硒亚甲基锁核酸 (SeLNA) 修饰的寡核苷酸
DOI：
10.1039/c2cc36464f
复制
发表时间：
2012
影响因子：
4.9
通讯作者：
Megan Wheeler
中科院分区：
文献类型：
--
作者：
Yamori W.;Masumoto C.;Fukayama H.;Makino A.;Megan Wheeler
研究了SeLNA核苷酸的酶识别。发现KOD XL DNA聚合酶是引物延伸反应中的有效酶。SeLNA修饰的DNA模板的聚合酶链反应（PCR）扩增也有效地实现了通过purision和KOD XL DNA聚合酶。
Enzymatic recognition of SeLNA nucleotides was investigated. KOD XL DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of SeLNA-modified DNA templates was also efficiently achieved by Phusion and KOD XL DNA polymerases.