Feasibility Study on Quantitative Measurements of Singlet Oxygen Generation Using Singlet Oxygen Sensor Green
Feasibility Study on Quantitative Measurements of Singlet Oxygen Generation Using Singlet Oxygen Sensor Green
复制标题
使用单线态氧传感器绿色定量测量单线态氧产生的可行性研究
DOI：
10.1007/s10895-012-1114-5
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发表时间：
2013-01-01
影响因子：
2.7
通讯作者：
Xie, Shusen
中科院分区：
文献类型：
--
作者：
Lin, Huiyun;Shen, Yi;Xie, Shusen
本研究的目的是探讨利用一种新开发的单重态氧（O-1（2））特异性荧光探针--单重态氧传感器绿色试剂（SOSG）定量测定O-1（2）生成的可行性。使用SOSG间接监测初始空气稳定磷酸盐缓冲盐水（PBS）中模型光敏剂虎红（RB）光辐照产生的O-1（2）。在O-1（2）存在下，SOSG能与O-1（2）反应生成SOSG内过氧化物（SOSG EP），SOSG EP能发出强的绿色荧光，其最大吸收波长为531 nm。SOSG-EP的绿色荧光主要取决于RB和SOSG的初始浓度以及光照射时间。此外，RB敏化的SOSG的光氧化的动力学分析进行，第一次，允许直接从反应速率的测定O-1（2）生成的定量测量。此外，用SOSG法测得的卟啉类光敏剂血卟啉单甲醚（HMME）在PBS中的O-1（2）量子产率与直接测量O-1（2）发光法测得的值吻合较好。本研究的结果清楚地表明，使用SOSG的O-1（2）生成的定量测量可以通过使用适当的测量方案确定反应速率来实现。
The purpose of this study is to investigate the feasibility for quantitative measurement of singlet oxygen (O-1(2)) generation by using a newly developed O-1(2)-specific fluorescence probe Singlet Oxygen Sensor Green reagent (SOSG). O-1(2) generation from photoirradiation of a model photosensitizer Rose Bengal (RB), in initially air-statured phosphate buffered saline (PBS) was indirectly monitored with SOSG. In the presence of O-1(2), SOSG can react with O-1(2) to produce SOSG endoperoxides (SOSG-EP) that emit strong green fluorescence with the maximum at 531 nm. The green fluorescence of SOSG-EP is mainly dependent on the initial concentrations of RB and SOSG, and the photoirradiation time for O-1(2) generation. Furthermore, kinetic analysis of the RB-sensitized photooxidation of SOSG is performed that, for the first time, allows quantitative measurement of O-1(2) generation directly from the determination of reaction rate. In addition, the obtained O-1(2) quantum yield of porphyrin-based photosensitizer hematoporphyrin monomethyl ether (HMME) in PBS by using SOSG is in good agreement with the value that independently determined by using direct measurement of O-1(2) luminescence. The results of this study clearly demonstrate that the quantitative measurement of O-1(2) generation using SOSG can be achieved by determining the reaction rate with an appropriate measurement protocol.