IDENTIFICATION AND CHARACTERIZATION OF THE BACILLUS-SUBTILIS SPOIIP LOCUS
IDENTIFICATION AND CHARACTERIZATION OF THE BACILLUS-SUBTILIS SPOIIP LOCUS
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DOI：
10.1128/jb.177.3.716-722.1995
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发表时间：
1995-02-01
影响因子：
3.2
通讯作者：
STRAGIER, P
中科院分区：
文献类型：
--
作者：
FRANDSEN, N;STRAGIER, P
我们在枯草杆菌染色体紧邻gpr基因下游的区域中发现了一个额外的孢子形成基因，名为spoIIP（基因图谱上的227度），spoIIP的无效突变在吞噬的早期阶段阻止孢子形成（阶段IIii），一种与已经描述的spoIID和spoIIM突变体相似的表型，该基因编码401个残基的多肽，其被预测锚定在膜中，大部分蛋白质位于细胞质外。spoIIP基因由位于gpr和spoIIP阅读框之间的启动子转录。该启动子具有sigma（E）依赖性启动子的结构和遗传特征。spoIIP的转录被编码sigma（E）的基因spoIIGB中的突变消除，并且可以在工程化以产生活性形式的sigma（E）的细胞中的指数生长期间被诱导：在孢子形成过程中导致遗传嵌合体形成的质粒整合-切除实验表明，与SpoIID和SpoIIM一样，SpoIIP仅在母细胞中需要，spoIIP的破坏对sigma（F）和sigma（E）控制的调节子的表达几乎没有影响，但抑制了sigma（G）的转录。依赖性启动子的转录，并消除了启动子在sigma（K）控制下的转录，我们提出，SpoIIP蛋白与SpoIID和SpoIIM一起参与了位于孢子形成隔膜中的肽聚糖的溶解。
We have identified an additional sporulation gene, named spoIIP, in the region of the Bacillus subtilis chromosome located immediately downstream of the gpr gene (227 degrees on the genetic map), A null mutation of spoIIP arrests sporulation at an early stage of engulfment (stage IIii), a phenotype similar to that already described for spoIID and spoIIM mutants, This gene encodes a 401-residue polypeptide, which is predicted to be anchored in the membrane, most of the protein being localized outside the cytoplasm, The spoIIP gene is transcribed from a promoter located in the interval between the gpr and the spoIIP reading frames. This promoter has the structural and genetic characteristics of a sigma(E)-dependent promoter. Transcription of spoIIP is abolished by a mutation in spoIIGB, the gene encoding sigma(E), and can be induced during exponential growth in cells engineered to produce an active form of sigma(E) :Plasmid integration-excision experiments leading to the formation of genetic mosaics during sporulation indicate that as with SpoIID and SpoIIM, SpoIIP is required only in the mother cell, Disruption of spoIIP had little or no effect on the expression of sigma(F)- and sigma(E)-controlled regulons but inhibited transcription from sigma(G)-dependent promoters and abolished transcription from promoters under the control of sigma(K), We propose that, together with SpoIID and SpoIIM, the SpoIIP protein is involved in the dissolution of the peptidoglycan located in the sporulation septum.