ACTIN, ALPHA-ACTININ, AND VINCULIN ARE ASSOCIATED WITH SEPTATE JUNCTIONS IN INSECTA
ACTIN, ALPHA-ACTININ, AND VINCULIN ARE ASSOCIATED WITH SEPTATE JUNCTIONS IN INSECTA
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DOI：
10.1002/cm.970260304
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发表时间：
1993-01-01
影响因子：
--
通讯作者：
CAMATINI, M
中科院分区：
文献类型：
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作者：
COLOMBO, A;BONFANTI, P;CAMATINI, M
本文报道了天蛾幼虫（昆虫纲，鳞翅目）中肠柱状细胞与光滑隔连接处的细胞骨架成分的特征。肌球蛋白亚片段1的装饰和肌动蛋白的免疫染色表明，与分隔连接的细丝构成的肌动蛋白。此外，使用免疫化学和免疫定位技术相结合，证据表明，α-辅肌动蛋白，肌球蛋白II，和黏着斑蛋白本地化靠近专门的质膜。微丝束插入到膜下的F-肌动蛋白/α-辅肌动蛋白/黏着斑蛋白复合物，以前描述的脊椎动物连接的adherens类型，似乎是一个更一般的组织，包括昆虫分隔连接在这里检查。(C)1993 Wiley-Liss，Inc.
Cytoskeletal elements associated with the smooth septate junctions linking the midgut columnar cells of Manduca sexta larvae (Insecta, Lepidoptera) were characterized. Myosin subfragment 1 decoration and immunostaining for actin demonstrated that the filaments associated with the septate junctions were constituted of actin. Moreover, using a combination of immunochemical and immunolocalization techniques, evidence is presented that alpha-actinin, myosin II, and vinculin are localized close to the specialized plasma membranes. The insertion of microfilament bundles into submembranous F-actin/alpha-actinin/vinculin complexes, previously described in vertebrate junctions of adherens type, appears to be a more general organization, including the insect septate junction here examined. (C) 1993 Wiley-Liss, Inc.