Novel domain arrangement in the crystal structure of a truncated acetyl-CoA synthase from Moorella thermoacetica.
Novel domain arrangement in the crystal structure of a truncated acetyl-CoA synthase from Moorella thermoacetica.
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来自热乙酸摩尔菌的截短的乙酰辅酶A合酶晶体结构中的新结构域排列。
DOI：
10.1021/bi9003952
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发表时间：
2009
期刊：
影响因子：
2.9
通讯作者：
Fontecilla-Camps,JuanC
中科院分区：
文献类型：
--
作者：
Volbeda,Anne;Darnault,Claudine;Tan,Xiangshi;Lindahl,PaulA;Fontecilla-Camps,JuanC
Ni-dependent acetyl-CoA synthase (ACS) and CO dehydrogenase (CODH) constitute the central enzyme complex of the Wood−Ljungdahl pathway of acetyl-CoA formation. The crystal structure of a recombinant bacterial ACS lacking the N-terminal domain that interacts with CODH shows a large reorganization of the remaining two globular domains, producing a narrow cleft of suitable size, shape, and nature to bind CoA. Sequence comparisons with homologous archaeal enzymes that naturally lack the N-terminal domain show that many amino acids lining this cleft are conserved. Besides the typical [4Fe-4S] center, the A-cluster contains only one proximal metal ion that, according to anomalous scattering data, is most likely Cu or Zn. Incorporation of a functional Ni2Fe4S4A-cluster would require only minor structural rearrangements. Using available structures, a plausible model of the interaction between CODH and the smaller ACS in archaeal multienzyme complexes is presented, along with a discussion of evolutionary relationships of the archaeal and bacterial enzymes.
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平衡透析研究以及辅酶 A 与热乙酸梭菌乙酰辅酶 A 合酶/一氧化碳脱氢酶结合的机制意义。
DOI：
10.1007/s007750050346
发表时间：
1999
期刊：
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry
影响因子：
--
作者：
Wilson,BE;Lindahl,PA
通讯作者：
Lindahl,PA
影响因子：
15
作者：
Barondeau, DP;Lindahl, PA
通讯作者：
Lindahl, PA
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
A. Mccoy;R. Grosse-Kunstleve;L. C. Storoni;R. Read
通讯作者：
A. Mccoy;R. Grosse-Kunstleve;L. C. Storoni;R. Read
嗜热甲烷八叠球菌的乙酰辅酶 A 脱羰酶/合酶复合体的 A 簇：Ni 和 Fe K-Edge XANES 和 EXAFS Chem。
影响因子：
15
作者：
W. Gu;S. Gencic;A. Cramer;D. Grahame
通讯作者：
D. Grahame
DOI：
10.1107/s0907444903006516
发表时间：
2003-05-01
影响因子：
2.2
作者：
Diederichs, K;McSweeney, S;Ravelli, RBG
通讯作者：
Ravelli, RBG