Could an increase in airway smooth muscle shortening velocity cause airway hyperresponsiveness?
Could an increase in airway smooth muscle shortening velocity cause airway hyperresponsiveness?
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DOI：
10.1152/ajplung.00228.2010
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发表时间：
2011-01-01
影响因子：
4.9
通讯作者：
Lauzon, Anne-Marie
中科院分区：
文献类型：
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作者：
Bullimore, Sharon R.;Siddiqui, Sana;Lauzon, Anne-Marie
Bullimore SR, Siddiqui S, Donovan GM, Martin JG, Sneyd J, Bates JH, Lauzon A. Could an increase in airway smooth muscle shortening velocity cause airway hyperresponsiveness? Am J Physiol Lung Cell Mol Physiol 300: L121-L131, 2011. First published October 22, 2010; doi:10.1152/ajplung.00228.2010.-Airway hyperresponsiveness (AHR) is a characteristic feature of asthma. It has been proposed that an increase in the shortening velocity of airway smooth muscle (ASM) could contribute to AHR. To address this possibility, we tested whether an increase in the isotonic shortening velocity of ASM is associated with an increase in the rate and total amount of shortening when ASM is subjected to an oscillating load, as occurs during breathing. Experiments were performed in vitro using 27 rat tracheal ASM strips supramaximally stimulated with methacholine. Isotonic velocity at 20% isometric force (Fiso) was measured, and then the load on the muscle was varied sinusoidally (0.33 +/- 0.25 Fiso, 1.2 Hz) for 20 min, while muscle length was measured. A large amplitude oscillation was applied every 4 min to simulate a deep breath. We found that: 1) ASM strips with a higher isotonic velocity shortened more quickly during the force oscillations, both initially (P < 0.001) and after the simulated deep breaths (P = 0.002); 2) ASM strips with a higher isotonic velocity exhibited a greater total shortening during the force oscillation protocol (P < 0.005); and 3) the effect of an increase in isotonic velocity was at least comparable in magnitude to the effect of a proportional increase in ASM force-generating capacity. A cross-bridge model showed that an increase in the total amount of shortening with increased isotonic velocity could be explained by a change in either the cycling rate of phosphorylated cross bridges or the rate of myosin light chain phosphorylation. We conclude that, if asthma involves an increase in ASM velocity, this could be an important factor in the associated AHR.
