Impact of Phanerochaete chrysosporium inoculation on indigenous bacterial communities during agricultural waste composting
Impact of Phanerochaete chrysosporium inoculation on indigenous bacterial communities during agricultural waste composting
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DOI：
10.1007/s00253-012-4124-y
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发表时间：
2013-04
影响因子：
5
通讯作者：
Jiachao Zhang;G. Zeng;Yaoning Chen;Man Yu;Hongli Huang;Changzheng Fan;Yi Zhu;Hui Li;Zhifeng Liu;Ming Chen;Min Jiang
中科院分区：
文献类型：
--
作者：
Jiachao Zhang;G. Zeng;Yaoning Chen;Man Yu;Hongli Huang;Changzheng Fan;Yi Zhu;Hui Li;Zhifeng Liu;Ming Chen;Min Jiang
本研究旨在区分农业废弃物堆肥过程中接种黄孢原毛革菌的单独效果和不同接种方式对土壤细菌群落的异质性。在不同时期接种黄孢原毛平革菌。采用定量PCR和变性梯度凝胶电泳分析方法测定细菌群落的丰度和结构。结果表明，接种黄孢原毛平革菌对细菌群落丰度有明显的促进作用。细菌群落丰度与堆温、铵态氮、硝态氮含量显著相关（P<0.006）。方差分配分析表明，接种黄孢原毛平革菌可直接解释20.5%（P=0.048）的细菌群落变异，而不同接种方式引起的样品性质变化可间接解释35.1%（P=0.002）的细菌群落变异。接种黄孢原毛平革菌后，菌群结构与堆体温度、水溶性碳（WSC）和C/N比显著相关。C/N仅能解释群落结构变异的7.9%（P=0.03），堆温和WSC分别能解释群落结构变异的7.7%（P=0.026）和7.5%（P=0.034）。接种黄孢原毛平革菌可能通过提高堆体温度、提高基质利用率和改变其他理化因子间接影响土著细菌群落。
This research was conducted to distinguish between the separate effects of the Phanerochaete chrysosporium inoculation and sample property heterogeneity induced by different inoculation regimes on the indigenous bacterial communities during agricultural waste composting. P. chrysosporium was inoculated during different phases. The bacterial community abundance and structure were determined by quantitative PCR and denaturing gradient gel electrophoresis analysis, respectively. Results indicated a significant stimulatory effect of P. chrysosporium inoculation on the bacterial community abundance. The bacterial community abundance significantly coincided with pile temperature, ammonium, and nitrate (P<0.006). Variance partition analysis showed that the P. chrysosporium inoculation directly explained 20.5% (P=0.048) of the variation in the bacterial communities, whereas the sample property changes induced by different inoculation regimes indirectly explained up to 35.1% (P=0.002). The bacterial community structure was significantly related to pile temperature, water-soluble carbon (WSC), and C/N ratio when P. chrysosporium were inoculated. The C/N ratio solely explained 7.9% (P=0.03) of the variation in community structure, whereas pile temperature and WSC explained 7.7% (P=0.026) and 7.5% (P=0.034) of the variation, respectively. P. chrysosporium inoculation affected the indigenous bacterial communities most probably indirectly through increasing pile temperature, enhancing the substrate utilizability, and changing other physico-chemical factors.