Non-propagative human parainfluenza virus type 2 nasal vaccine robustly protects the upper and lower airways against SARS-CoV-2.
Non-propagative human parainfluenza virus type 2 nasal vaccine robustly protects the upper and lower airways against SARS-CoV-2.
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DOI：
10.1016/j.isci.2021.103379
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发表时间：
2021-12-17
期刊：
影响因子：
5.8
通讯作者：
Nosaka T
中科院分区：
文献类型：
--
作者：
Ohtsuka J;Imai M;Fukumura M;Maeda M;Eguchi A;Ono R;Maemura T;Ito M;Yamayoshi S;Kataoka Y;Kawaoka Y;Nosaka T
利用复制能力不强的人副流感病毒2型(HPIV2)载体BC-PIV，以稳定的RNA和外膜蛋白的形式携带异位基因，研制了SARS-CoV-2鼻腔疫苗。表达SARS-CoV-2毒株S-2 PM株K986P/V987P的BC-PIV具有冠状病毒囊膜。鼻腔接种BC-PIV/S-2 PM后，小鼠产生高水平的中和免疫球蛋白G和粘膜抗S蛋白的抗体。尽管BC-PIV具有血凝活性，但BC-PIV/S-2 PM缺乏这种活性，这与病毒表面存在大量的刺突蛋白相一致。此外，单剂鼻腔接种BC-PIV/S-2 PM疫苗在免疫后11周完全保护了仓鼠的肺部免受SARS-CoV-2的感染，并在挑战前两周加强了疫苗接种，几乎完全保护了鼻甲免受SARS-CoV-2的感染。因此，这种hPIV2/Spike嵌合鼻腔疫苗是一种有希望的SARS-CoV-2疫苗候选疫苗，以遏制病毒传播。针对Spike的非复制型病毒载体诱导黏膜免疫以阻断感染病毒载体携带Spike蛋白的包膜具有冠状结构仓鼠一次鼻接种完全保护肺部免受严重急性呼吸综合征冠状病毒2(SARS-CoV-2)的感染仓鼠两次鼻接种几乎完全保护上呼吸道感染控制在卫生技术中；病毒学
We developed an intranasal vaccine against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using the replication-incompetent human parainfluenza virus type 2 (hPIV2) vector BC-PIV, which can deliver ectopic gene as stable RNA and ectopic protein on the envelope. BC-PIV expressing the full-length prefusion-stabilized spike gene (K986P/V987P) of SARS-CoV-2, S-2PM, possessed a corona-like viral envelope. Intranasal vaccination of mice with BC-PIV/S-2PM induced high levels of neutralizing immunoglobulin G (IgG) and mucosal IgA antibodies against the spike protein. Although BC-PIV showed hemagglutinating activity, BC-PIV/S-2PM lacked such activity, in accordance with the presence of the massive spike protein on the viral surface. Furthermore, single-dose intranasal vaccination of hamsters with BC-PIV/S-2PM completely protected the lungs from SARS-CoV-2 at 11-week post-immunization, and boost vaccination two weeks before the challenge conferred virtually complete protection of the nasal turbinates against SARS-CoV-2. Thus, this chimeric hPIV2/spike intranasal vaccine is a promising vaccine candidate for SARS-CoV-2 to curtail virus transmission. Non-replicating viral vector against spike induces mucosal immunity to block infection The viral vector carries spike protein on its envelope with corona-like structure One-shot nasal vaccination of hamsters completely protects lungs against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Two-shot nasal vaccination of hamsters nearly completely protects the upper airway Infection control in health technology; Virology
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DOI：
10.1056/nejmoa2035389
发表时间：
2021-02-04
期刊：
The New England journal of medicine
影响因子：
--
作者：
Baden LR;El Sahly HM;Essink B;Kotloff K;Frey S;Novak R;Diemert D;Spector SA;Rouphael N;Creech CB;McGettigan J;Khetan S;Segall N;Solis J;Brosz A;Fierro C;Schwartz H;Neuzil K;Corey L;Gilbert P;Janes H;Follmann D;Marovich M;Mascola J;Polakowski L;Ledgerwood J;Graham BS;Bennett H;Pajon R;Knightly C;Leav B;Deng W;Zhou H;Han S;Ivarsson M;Miller J;Zaks T;COVE Study Group
通讯作者：
COVE Study Group
影响因子：
6.4
作者：
Li, Kun;Li, Zhuo;He, Biao
通讯作者：
He, Biao
DOI：
10.1126/science.1243283
发表时间：
2013-11-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
McLellan JS;Chen M;Joyce MG;Sastry M;Stewart-Jones GB;Yang Y;Zhang B;Chen L;Srivatsan S;Zheng A;Zhou T;Graepel KW;Kumar A;Moin S;Boyington JC;Chuang GY;Soto C;Baxa U;Bakker AQ;Spits H;Beaumont T;Zheng Z;Xia N;Ko SY;Todd JP;Rao S;Graham BS;Kwong PD
通讯作者：
Kwong PD
影响因子：
5.4
作者：
Bolles, Meagan;Deming, Damon;Baric, Ralph S.
通讯作者：
Baric, Ralph S.
DOI：
10.1073/pnas.2106535118
发表时间：
2021-07-06
影响因子：
11.1
作者：
Imai M;Halfmann PJ;Yamayoshi S;Iwatsuki-Horimoto K;Chiba S;Watanabe T;Nakajima N;Ito M;Kuroda M;Kiso M;Maemura T;Takahashi K;Loeber S;Hatta M;Koga M;Nagai H;Yamamoto S;Saito M;Adachi E;Akasaka O;Nakamura M;Nakachi I;Ogura T;Baba R;Fujita K;Ochi J;Mitamura K;Kato H;Nakajima H;Yagi K;Hattori SI;Maeda K;Suzuki T;Miyazato Y;Valdez R;Gherasim C;Furusawa Y;Okuda M;Ujie M;Lopes TJS;Yasuhara A;Ueki H;Sakai-Tagawa Y;Eisfeld AJ;Baczenas JJ;Baker DA;O'Connor SL;O'Connor DH;Fukushi S;Fujimoto T;Kuroda Y;Gordon A;Maeda K;Ohmagari N;Sugaya N;Yotsuyanagi H;Mitsuya H;Suzuki T;Kawaoka Y
通讯作者：
Kawaoka Y