ACIDIC THIOL PROTEINASE ACTIVITY OF SCHISTOSOMA-MANSONI CULTURED INVITRO
ACIDIC THIOL PROTEINASE ACTIVITY OF SCHISTOSOMA-MANSONI CULTURED INVITRO
复制标题
DOI：
10.1016/0166-6851(82)90054-8
复制
发表时间：
1982-01-01
影响因子：
1.5
通讯作者：
BASCH, PF
中科院分区：
文献类型：
--
作者：
DRESDEN, MH;PAYNE, DC;BASCH, PF
使用荧光底物分析体外培养12天至33周的曼氏梭菌的蛋白酶活性，并将其与感染小鼠的成年曼氏梭菌进行比较。测定的酶活性是先前描述的酸性巯基依赖性蛋白酶。蛋白酶活性在发育早期出现，并迅速上升，占总可提取蠕虫蛋白的比例约为恒定。培养的蠕虫比体内发育的蠕虫的比活性高5-20倍。每条人工培养的蠕虫和体内蠕虫的活性大致相当。
S. mansoni cultured in vitro for periods from 12 days to 33 wk were analyzed for proteinase activity using a fluorescent substrate and compared with adult S. mansoni worms from infected mice. The enzyme activity measured was that of an acidic thiol-dependent proteinase previously described. Proteinase activity appears early in development and rises rapidly to an approximately constant proportion of the total extractable worm protein. Cultured worms have 5-20 times higher specific activity than in vivo-developed worms. Cultured worms and in vivo worms have approximately equivalent activities per worm.