Generation of a photocontrollable recombinant bovine parainfluenza virus type 3
Generation of a photocontrollable recombinant bovine parainfluenza virus type 3
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DOI：
10.1111/1348-0421.13052
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发表时间：
2023-01-22
影响因子：
2.6
通讯作者：
Takeda，Makoto
中科院分区：
文献类型：
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作者：
Okura，Takashi;Tahara，Maino;Takeda，Makoto
牛副流感病毒3型（BPIV 3）是一种很有前途的疫苗载体，可预防各种呼吸道病毒感染，包括人PIV 3、呼吸道合胞病毒和严重急性呼吸综合征-冠状病毒2型感染。在这项研究中，我们结合磁铁系统和反向遗传方法，以产生光可控BPIV 3。在BPIV 3大蛋白基因的H2区插入了一个光学可变的磁性基因，该基因编码一种依赖于RNA的RNA聚合酶。所产生的光可控BPIV 3仅在用蓝光照射时才在细胞片的特定区域生长，这表明时空控制可以帮助BPIV 3的安全临床应用。
Bovine parainfluenza virus type 3 (BPIV3) is a promising vaccine vector against various respiratory virus infections, including the human PIV3, respiratory syncytial virus, and severe acute respiratory syndrome‐coronavirus 2 infections. In this study, we combined the Magnet system and reverse genetic approach to generate photocontrollable BPIV3. An optically controllableMagnetgene was inserted into the H2 region of the BPIV3large proteingene, which encodes an RNA‐dependent RNA polymerase. The generated photocontrollable BPIV3 grew in specific regions of the cell sheet only when illuminated with blue light, suggesting that spatiotemporal control can aid in safe clinical applications of BPIV3.