Dynamic expression patterns of leucine-rich repeat containing protein 10 in the heart
Dynamic expression patterns of leucine-rich repeat containing protein 10 in the heart
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DOI：
10.1002/dvdy.21225
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发表时间：
2007-08-01
影响因子：
2.5
通讯作者：
Lee, Youngsook
中科院分区：
文献类型：
--
作者：
Kim, Ki-Hyun;Kim, Tae-Gyun;Lee, Youngsook
富含亮氨酸重复序列的蛋白 10 (LRRC10) 是一种心脏特异性因子，其功能尚不清楚。检查基因产物的细胞内位置是确定蛋白质生物学功能的关键步骤。我们对小鼠的表达分析表明，LRRC10 只在早期胚胎的心前区到成年心脏中表达。 LRRC10 表达在出生后显着升高，表明其在胚胎和成人心脏中的作用。有趣的是，LRRC10 在心肌细胞中表现出动态的细胞内表达模式。来自胚胎和新生儿的心肌细胞显示出弥漫性 LRRC10 细胞质和核染色。相比之下，在成年心肌细胞中观察到醒目的条纹，通过双重免疫染色，这些条纹与 Z 线、肌浆网 (SR) 和横管 (T) 管的标记物共定位。通过电子显微照片进行的进一步研究将 LRRC 10 置于二元组区域，其中 SR 与沿 Z 线定位的 T 管相互作用。
Leucine-rich repeat containing protein 10 (LRRC10) is a heart-specific factor whose function remains unknown. Examination of the intracellular location of the gene products is a critical step in determining the biological functions of the protein. Our expression analyses in mice indicate that LRRC10 is exclusively expressed from the precardiac region in early embryos to the adult heart. LRRC10 expression is markedly elevated upon birth, suggesting its role in the embryonic as well as adult hearts. Of interest, LRRC10 exhibits dynamic intracellular expression patterns in cardiomyocytes. Cardiomyocytes from embryos and newborns show diffuse cytoplasmic and nuclear staining of LRRC10. In contrast, striking striations are observed in adult cardiomyocytes, which are colocalized with the markers for the Z-line, sarcoplasmic reticulum (SR), and transverse (T)-tubule by double immunostaining. Further investigation by electron micrographs places LRRC 10 in a diad region where the SR interacts with the T-tubule that locates along the Z-line.