Evidence for the existence of α- and β-adrenoceptors on cultured glial cells—an electrophysiological study
Evidence for the existence of α- and β-adrenoceptors on cultured glial cells—an electrophysiological study
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培养的神经胶质细胞上存在 α- 和 β- 肾上腺素受体的证据——一项电生理学研究
DOI：
10.1016/0306-4522(82)90109-9
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发表时间：
1982
期刊：
影响因子：
3.3
通讯作者：
T. W. Lutz
中科院分区：
文献类型：
--
作者：
L. Hösli;E. Hösli;C. Zehntner;R. Lehmann;T. W. Lutz
本文研究了肾上腺素能α-和β-激动剂和拮抗剂对培养的大鼠中枢神经系统胶质细胞膜电位和电阻的作用。去甲肾上腺素和α-肾上腺素受体激动剂苯肾上腺素和可乐定（10− 7至10− 4 M）使神经胶质细胞膜去极化，而β-激动剂异丙肾上腺素在低浓度（10− 7和10− 6 M）下引起超极化。去甲肾上腺素和苯肾上腺素的作用可被α受体阻断剂酚妥拉明可逆地阻断，而异丙肾上腺素的作用可被β受体阻断剂阿替洛尔可逆地阻断。阿替洛尔不影响去甲肾上腺素的去极化作用。α-受体激动剂引起的胶质细胞去极化与氨基酸递质引起的不同，不是细胞外K+浓度变化的结果，结合生物化学和放射自显影结合研究结果，提示胶质细胞上存在α-和β-肾上腺素能受体，胶质细胞去极化由α-受体介导，而超极化则由β-受体激活引起。
The action of adrenergic α- and β-agonists and antagonists has been studied on the membrane potential and resistance of glial cells of cultured rat central nervous system. Noradrenaline and the α-adrenoceptor stimulating agents phenylephrine and clonidine (10−7to 10−4M) depolarized the glial membrane, whereas the β-agonist isoprenaline caused a hyperpolarization at low concentrations (10−7and 10−6M). The effects of noradrenaline and phenylephrine were reversibly blocked by the α-antagonist phentolamine, whereas those of isoprenaline were antagonized by the β-blocker atenolol. Atenolol did not affect the depolarization by noradrenaline. The glial depolarization induced by the α-agonists was not the consequence of a change in the extracellular K+-concentration unlike that produced by amino acid transmitters.The present results, together with those of biochemical and autoradiographic binding studies, suggest that α- and β-adrenoceptors occur on glial cells and that the glial depolarization is mediated by α-receptors, whereas the hyperpolarization is due to activation of β-receptors.
(-)-[125I]-碘吲哚洛尔，一种新型高选择性放射性碘标记β-肾上腺素能受体拮抗剂：测量完整大鼠星形细胞瘤细胞上的β-受体。
DOI：
--
发表时间：
1980
期刊：
Journal of cyclic nucleotide research
影响因子：
--
作者：
Barovsky,K;Brooker,G
通讯作者：
Brooker,G