Molecular cloning of a gene encoding translation initiation factor (TIF) from Candida albicans.
Molecular cloning of a gene encoding translation initiation factor (TIF) from Candida albicans.
复制标题
白色念珠菌编码翻译起始因子 (TIF) 的基因的分子克隆。
DOI：
10.1080/02681219680000701
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Nozawa,Y
中科院分区：
文献类型：
--
作者：
Mirbod,F;Nakashima,S;Kitajima,Y;Ghannoum,MA;Cannon,RD;Nozawa,Y
The differential display technique was applied to compare mRNAs from two clinical isolates ofCandida albicanswith different virulence; high (potent strain, 16240) and low (weak strain, 18084) extracellular phospholipase activities. Complementary DNA fragments corresponding to several apparently differentially expressed mRNAs were recovered and sequenced. A complementary DNA fragment seen distinctly in the potent phospholipase producing strain was highly homologous to the yeast translation initiation factor (TIF). The selected DNA fragment was then used as a probe to isolate its corresponding complementary DNA clone from a library ofC. albicansgenomic DNA. The sequence of isolated gene revealed an open reading frame of 1194 nucleotides with the potential to encode a protein of 397 amino acids with a predicted molecular weight of 43 kDa. Over its entire length, the amino acid sequence showed strong homology (78–89%) toSaccharomyces cerevisiae TIFand (63–80%) to mouse eIF-4A proteins. Therefore, ourC. albicansgene was identified to beTIF(Ca TIF). Northern blot analysis in the two strains ofC. albicansrevealed thatCa TIFexpression is 1·5-fold higher in the potent phospholipase producing strain. The restriction endonuclease digestion of genomic DNA from this potent strain revealed at least two hybridized bands in Southern blot analysis, suggesting two or more closely related sequences in theC. albicansgenome.
