Effects of Redox Mediators on Anaerobic Degradation of Phenol by Shewanella sp. XB
Effects of Redox Mediators on Anaerobic Degradation of Phenol by Shewanella sp. XB
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DOI：
10.1007/s12010-015-1490-9
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发表时间：
2015-01
影响因子：
3
通讯作者：
Jing Wang;Yang Zhou;Pei-qin Li;Hong Lu;Ruofei Jin;Guangfei Liu
中科院分区：
文献类型：
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作者：
Jing Wang;Yang Zhou;Pei-qin Li;Hong Lu;Ruofei Jin;Guangfei Liu
到目前为止，还没有关于氧化还原介体在无外源电子受体的苯酚厌氧降解中的作用的信息。介体对希瓦氏菌厌氧降解苯酚的影响。本研究首次对XB进行了研究。结果表明，在发酵条件下，加入0.20 mM蒽醌-2-磺酸盐（AQS）、0.05 mM蒽醌-2，6-二磺酸盐（AQDS）和0.05 mM核黄素，培养3 d，苯酚的去除率分别提高了2.87、3.31和2.89倍。进一步的分析发现，苯酚通过挥发性脂肪酸（VFA）作为中间体（特别是乙酸盐）发酵，并进一步转化为CH4。在反硝化条件下，当AQS、AQDS和核黄素分别加入反应体系中时，苯酚的去除效率分别提高了3.92、4.58和3.57倍。在此过程中，苯酚优先降解为CO2和H2O，而不是CH4。这些结果表明，这三种介质不仅显着提高苯酚的生物降解速率在发酵条件下，但也显着加速硝酸盐生物还原耦合苯酚降解。这些研究结果有助于开发有效的苯酚生物降解策略，更好地了解苯酚在自然环境中的命运。
Up to now, no information is available on the role of redox mediators in anaerobic degradation of phenol without exogenous electron acceptors. The effects of mediators on anaerobic biodegradation of phenol byShewanellasp. XB were investigated for the first time in this study. The results showed that the removal efficiencies of phenol were enhanced by 2.87-, 3.31-, and 2.89-fold under fermentation conditions in the presence of 0.20 mM anthraquinone-2-sulfonate (AQS), 0.05 mM anthraquinone-2,6-disulfonate (AQDS), and 0.05 mM riboflavin, respectively, in 3-day incubation. Further analysis found that phenol was fermented via volatile fatty acids (VFAs) as intermediates, particularly acetate, and further converted to CH4. Under denitrification conditions, phenol removal efficiencies were enhanced by 3.92-, 4.58-, and 3.57-fold when AQS, AQDS, and riboflavin, respectively, were added into reaction systems. During this process, phenol was preferentially degraded to CO2and H2O rather than CH4. These results indicate that the three mediators not only increased significantly phenol biodegradation rates under fermentation conditions, but also considerably accelerated nitrate bioreduction-coupled phenol degradation. These findings are beneficial to develop efficient strategies of phenol biodegradation and to better understand phenol fate in natural environments.