ASSOCIATION BETWEEN HISTAMINE-CONTAINING MAST-CELLS AND SENSORY NERVES IN THE SKIN AND AIRWAYS OF CONTROL AND CAPSAICIN-TREATED PIGS
ASSOCIATION BETWEEN HISTAMINE-CONTAINING MAST-CELLS AND SENSORY NERVES IN THE SKIN AND AIRWAYS OF CONTROL AND CAPSAICIN-TREATED PIGS
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DOI：
10.1007/bf00319042
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发表时间：
1991-06-01
影响因子：
3.6
通讯作者：
LUNDBERG, JM
中科院分区：
文献类型：
--
作者：
ALVING, K;SUNDSTROM, C;LUNDBERG, JM
研究了猪肥大细胞（常规染色和组胺免疫组化显示）与辣椒素敏感神经（含降钙素基因相关肽（CGRP）和P物质（SP））之间的关系。在1-乙基-3（3-二乙基氨基丙基）碳二亚胺（EDCDI）固定的皮肤组织中，在血管周围发现含组胺的肥大细胞和CGRP/ sp阳性神经密切相关。在edcdi固定气道粘膜中，仅检测到单个含组胺的肥大细胞。然而，发现许多阿利新蓝阳性肥大细胞，有时靠近气道上皮，其中含有丰富的CGRP/ sp神经。系统性辣椒素预处理2天后，CGRP/ sp神经纤维消失，组织肥大细胞、粒细胞、淋巴细胞数量和分布无变化，血白细胞数量无变化。将过敏原、组胺和辣椒素局部注射到蛔虫抗原致敏猪的皮肤中，可引起快速的轻度红斑（血管扩张）反应。过敏原和组胺也会导致血浆蛋白外渗，而辣椒素则不会。与没有耀斑相反，辣椒素处理的猪仍然发生蛋白质外渗反应。对蛔虫抗原的敏感性由ige样抗体介导。我们认为，猪皮肤中含有组胺的肥大细胞与辣椒素敏感感觉神经之间存在功能和形态上的关系。肥大细胞和感觉神经也存在于气道黏膜中，似乎与上皮密切相关。
The association between mast cells (visualized by routine staining and immunohistochemistry for histamine) and capsaicin-sensitive nerves (containing calcitonin gene-related peptide (CGRP) and substance P (SP)) was studied in the pig. In the 1-ethyl-3(3-diethylaminopropyl)carbodiimide (EDCDI)-fixed skin tissue, histamine-containing mast cells and CGRP/SP-positive nerves were found in close association around blood vessels. In the EDCDI-fixed airway mucosa, only single histamine-containing mast cells were detected. However, many alcian blue-positive mast cells were found, sometimes close to the airway epithelium where CGRP/SP-containing nerves were abundant. The CGRP/SP-containing nerve fibers were absent 2 days after systemic capsaicin pretreatment, but no changes in the number and distribution of tissue mast cells, granulocytes or lymphocytes, or the number of blood leukocytes were detected. Local injection of allergen, histamine and capsaicin into the skin of pigs actively sensitized with ascaris antigen caused a rapid light red-flare (vasodilation) reaction. Allergen and histamine, but not capsaicin, also produced plasma protein extravasation. In contrast to the absent flare, the protein extravasation response still occurred in capsaicin-treated pigs. The sensitivity to ascaris antigen was mediated by an IgE-like antibody. We conclude that a functional and morphological relationship exists between histamine-containing mast cells and capsaicin-sensitive sensory nerves in the pig skin. Mast cells and sensory nerves are also found in the airway mucosa and appear to be closely associated with the epithelium.