Comparisons of apomorphine-induced erection and spontaneous erection in rats by telemetric assessment of intracavernosal pressure
Comparisons of apomorphine-induced erection and spontaneous erection in rats by telemetric assessment of intracavernosal pressure
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DOI：
10.1111/andr.12004
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发表时间：
2015-03-01
期刊：
影响因子：
4.5
通讯作者：
Lee, S. W.
中科院分区：
文献类型：
--
作者：
Choo, S. H.;Sung, H. H.;Lee, S. W.
尽管有多种评估大鼠勃起功能的方法，但遥测监测的标准方法尚未建立。理论上，自发勃起（SE）的评估似乎是一种生理方法，但需要较长的测量时间和额外的努力。阿扑吗啡诱导勃起（AIE）是一种可行且简单的方法；然而，与 SE 的相关性尚未评估。我们使用海绵体内压 (ICP) 的遥测评估，比较了正常大鼠模型和两种疾病大鼠模型的 AIE 和 SE 勃起曲线。 7周龄雄性Sprague-Dawley大鼠被分为正常对照组、糖尿病（DM）组和高胆固醇血症（HC）组。 19周后，通过手术将遥测压力传感器（C40；数据科学）植入海绵体。一周后，腹腔注射阿朴吗啡（100g/kg）（AIE）或SE期间记录自由活动大鼠的ICP。性事件通过视觉识别和记录。仅分析性行为期间发生的压力增加。我们比较了勃起概况，例如持续时间、最大 ICP 和曲线下面积（AUC，时间 x ICP 曲线下面积）。双向方差分析显示测量方法对平均 AUC 没有显着影响（F-1，F-43=2.756，p 值=0.104），但不同疾病模型对平均 AUC 有显着影响（双向方差分析：F-2，F-43=12.929，p 值）
Although there are several methods for assessing erectile function in rats, the standard methods for telemetric monitoring have not been established. Theoretically assessment of spontaneous erection (SE) seems to be a physiologic method but it needs long measuring time and additional efforts. Apomorphine-induced erection (AIE) is one available and simple method; however, the correlation with SE has not been assessed. We compared erection profiles of AIE and SE in normal and two disease rat models using telemetric assessment of intracavernosal pressure (ICP). Seven-week-old male Sprague-Dawley rats were assigned to normal control, diabetes mellitus (DM) and hypercholesterolemia (HC) group. After 19weeks a telemetric pressure sensor (C40; Data Sciences) was surgically implanted in the corpus cavernosum. One week later, ICP was recorded in freely moving rats after intraperitoneal apomorphine (100g/kg) injection (AIE) or during SE. Sexual events were visually identified and recorded. Only the pressure increases that occurred during sexual behavior were analyzed. We compared the erectile profiles such as duration, maximal ICP and the area under the curve (AUC, area under time x ICP curves). Two-way anova revealed no significant effect of the measuring methods on the mean AUC (F-1,F-43=2.756, p-value=0.104), but a significant effect of different disease models on mean AUC (two-way anova: F-2,F-43=12.929, p-value