Evaluation of hydroxyapatite microspheres made from a borate glass to separate protein mixtures
Evaluation of hydroxyapatite microspheres made from a borate glass to separate protein mixtures
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DOI：
10.1007/s10853-008-2756-1
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发表时间：
2008-08-01
影响因子：
4.5
通讯作者：
Day, Delbert E.
中科院分区：
文献类型：
--
作者：
Han, Xue;Du, Ming;Day, Delbert E.
研究了从含钙硼酸盐玻璃转化而来的羟基磷灰石 (HA, Ca-10(PO4)(6)(OH)(2)) 的蛋白质吸附和色谱特性。通过将硼酸盐玻璃微球与 0.25 M 磷酸盐 (K2HPO4) 溶液在 37 摄氏度（pH 9.0）下反应 24 小时，将其转化为 HA。将直径为45-90μm的HA微球手工装入钢柱（4.6mm×80mm）中，用于分离牛血清白蛋白（BSA）和溶菌酶的二元蛋白质混合物。直径 < 45 针的 HA 微球用于分离 BSA、肌红蛋白和溶菌酶的蛋白质混合物。这些微球的直径比商业HA柱填充球形颗粒23μm大20-30倍，但这些微球具有六倍大的表面积和更均匀的球形。这些优点弥补了它们较大的尺寸，并且在分离所研究的蛋白质方面，分离结果与市售的 HA 柱相当。这些独特的 HA 微球由硼酸盐玻璃微球制成，被认为可用作色谱法中蛋白质分离的填充材料。
A hydroxyapatite (HA, Ca-10(PO4)(6)(OH)(2)), transformed from a calcium-containing borate glass, has been investigated for its protein adsorption and chromatographic characteristics. Microspheres of the borate glass were transformed into HA by reacting them with a 0.25 M phosphate (K2HPO4) solution for 24 h at 37 degrees C (pH 9.0). The HA microspheres with a diameter of 45-90 mu m were hand packed into a steel column (4.6 mm x 80 mm) and used to separate a binary protein mixture of bovine serum albumin (BSA) and lysozyme. HA microspheres, with a diameter < 45 pin, were used for separating a protein mixture of BSA, myoglobin, and lysozyme. These microspheres had a diameter that was 20-30 times larger than commercial HA column packing spherical particles, 23 mu m, but these microspheres had a six times larger surface area and a more uniform spherical shape. These advantages compensated for their larger size and the separation results were comparable to those commercially available HA columns in the separation of the proteins studied. These unique HA microspheres, made from microspheres of a borate glass, are considered to be useful as packing materials for protein separation in chromatography.