BREAKPOINT CLUSTERS OF THE PML GENE IN ACUTE PROMYELOCYTIC LEUKEMIA - PRIMARY STRUCTURE OF THE RECIPROCAL PRODUCTS OF THE PML-RARA GENE IN A PATIENT WITH T(15 17)
BREAKPOINT CLUSTERS OF THE PML GENE IN ACUTE PROMYELOCYTIC LEUKEMIA - PRIMARY STRUCTURE OF THE RECIPROCAL PRODUCTS OF THE PML-RARA GENE IN A PATIENT WITH T(15 17)
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DOI：
10.1002/gcc.2870060302
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发表时间：
1993-03-01
影响因子：
3.7
通讯作者：
CHEN, Z
中科院分区：
文献类型：
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作者：
DONG, S;GENG, JP;CHEN, Z
对急性早幼粒细胞白血病(APL)t(15；17)易位的DNA研究表明，17号染色体上的维甲酸受体α(RARA)基因与15号染色体上的PML基因并列。PML断裂点被定位到3个簇：bcr 1、bcr2和bcr3。我们对33例中国APL患者的PML断点分布进行了研究。22例患者在bcr 1内，2例在bcr2内，9例在bcr3内。一名患者和他们的正常对应者的相互染色体易位关节的一级结构已经确定，并与以前报道的两例进行了比较。这些研究揭示了断裂点附近可能的拓扑异构酶II裂解部位，并暗示了DNA附着部位对核基质的影响。我们认为这些特征与产生易位的非法重组过程有关。
DNA studies of the translocation t(15;17) in acute promyelocytic leukemia (APL) have shown that the retinoic acid receptor alpha (RARA) gene on chromosome 17 is juxtaposed to the promyelocytic leukemia (PML) gene on chromosome 15. The PML breakpoints have been mapped to 3 clusters: bcr 1, bcr2, and bcr3. We have examined the PML breakpoint distribution in a series of 33 Chinese patients with APL. Twenty-two patients fell within bcr 1, 2 within bcr2, and 9 within bcr3. The primary structure of the reciprocal chromosome translocation joints of one patient and that of their normal counterparts have been determined and compared to those of 2 previously reported cases. These studies revealed possible topoisomerase II cleavage sites close to the breakpoints and suggested implications of DNA attachment sites to nuclear matrix. We propose that these features are relevant to the process of illegitimate recombination generating the translocation.