Hepatocyte growth factor expression correlates with cyclooxygenase-2 pathway in human salivary gland tumors
Hepatocyte growth factor expression correlates with cyclooxygenase-2 pathway in human salivary gland tumors
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DOI：
10.1016/j.oraloncology.2005.06.012
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发表时间：
2006-01-01
期刊：
影响因子：
4.8
通讯作者：
Kaneko, A
中科院分区：
文献类型：
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作者：
Aoki, T;Tsukinoki, K;Kaneko, A
环氧化酶-2 （cycloxygenase -2, COX-2）在细胞增殖、肿瘤侵袭和抑制细胞凋亡中发挥重要作用。我们报道了COX-2在唾液腺瘤中的表达及其参与抑制细胞凋亡。COX-2可以诱导多种细胞因子和生长因子。另一方面，肝细胞生长因子（HGF）具有细胞迁移、增殖、血管生成和抑制凋亡等作用。近年来有报道称，HGF在唾液腺肿瘤的分化中也起作用。因此，我们从临床病理角度探讨COX-2表达是否影响HGF表达，并探讨COX-2通路在唾液腺肿瘤中的作用。组织样本为手术获得的多形性腺瘤（n = 40）。免疫组织化学检测COX-2表达和HGF表达。COX-2、HGF免疫染色强度为+3 ~ 0。采用Spearman秩相关法检测COX-2与HGF表达的相关性。此外，在给药0、10、100单位COX-2后培养唾液腺肿瘤细胞株HSG，使用Light Cycler System进行实时荧光定量PCR分析HGF mRNA的表达。在多形性腺瘤中，COX-2和HGF主要在腔内肿瘤细胞中表达，两者的表达位点几乎相同。COX-2免疫染色表达强度与HGF表达强度呈显著相关（p = 0.003, r = 0.457）。COX-2处理涎腺肿瘤细胞时，HGF mRNA表达量增加。提示COX-2的表达可能诱导唾液腺肿瘤中HGF的表达。(c) 2005 Elsevier Ltd版权所有。
It has been reported that cyclooxygenase-2 (COX-2) plays roles in cell proliferation, tumor invasion and inhibition of apoptosis. We reported COX-2 expression in salivary adenoma and its involvement in inhibition of apoptosis. COX-2 is known to induce a number of cytokines and growth factors. On the other hand, hepatocyte growth factor (HGF) plays roles such as cell migration, proliferation, angiogenesis and inhibition of apoptosis. Recently it has been reported that HGF also plays a role in tumor differentiation in salivary gland tumors. So, we investigated that whether COX-2 expression affected HGF expression clinicopathologically, and discussed the roles of COX-2 pathway in salivary gland tumors. Tissues samples were pleomorphic adenomas (n = 40) obtained surgically. COX-2 expression and HGF expression were examined immunohistochemically. Immunostaining intensities of COX-2 and HGF were classified as +3 to 0. Spearman's rank correlation was used to examine correlation of COX-2 and HGF expression. Furthermore, a salivary gland tumor cell line, HSG, was cultured after administration of either 0, 10, 100 units of COX-2 and quantification of HGF mRNA was analyzed by Real Time PCR using the Light Cycler System. In pleomorphic adenoma, COX-2 and HGF were mainly expressed in luminal tumor cells, and the sites of expression of the two were almost the same. Immunostaining intensity of COX-2 expression was significantly correlated with intensity of HGF expression (p = 0.003, r = 0.457). Moreover, the content of HGF mRNA expression increased when COX-2 was administered to salivary gland tumor cell tine HSG. It is suggested that COX-2 expression may induce HGF expression in salivary gland tumors. (c) 2005 Elsevier Ltd. All rights reserved.