Distribution of PLGA nanoparticles in chinchilla cochleae
Distribution of PLGA nanoparticles in chinchilla cochleae
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DOI：
10.1016/j.otohns.2007.04.013
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发表时间：
2007-10-01
影响因子：
3.4
通讯作者：
Balough, Ben J.
中科院分区：
文献类型：
--
作者：
Ge, Xianxi;Jackson, Ronald L.;Balough, Ben J.
目的：研究聚乳酸/羟基乙酸包封的氧化铁纳米颗粒（PLGA-NPs）贴敷在圆窗膜（RWM）上后在毛丝鼠耳蜗中的分布。在将PLGA-NP放置在RWM上40分钟后，从鼓阶抽出外淋巴。用2.5%戊二醛固定RWM和耳蜗，并进行透射电子显微镜检查。结果：在耳蜗中发现纳米颗粒，有或没有暴露于RWM，外淋巴，内淋巴中出现的磁力，并在Corti器官的多个位置。电子能量损失谱证实了铁元素nanoparticle.CONCLUSION：纳米粒子分布在整个内耳后，应用于灰鼠RWM，有和没有magneticforces. SIGNANCE：PLGA-NP应用于RWM可能有潜力的持续治疗内耳。(c)2007年美国耳鼻咽喉头颈外科学会基金会。All rights reserved.
OBJECTIVES: To study the distribution of polylactic/glycolic acid-encapsulated iron oxide nanoparticles (PLGA-NPs) in chinchilla cochleae after application on the round window membrane (RWM).STUDY DESIGN AND SETTING: Six chinchillas (12 ears) were equally divided into controls (no treatments) and experimentals (PLGA-NP with or without magnetic exposure). After 40 minutes of PLGA-NP placement on the RWM, perilymph was withdrawn from the scala tympani. The RWM and cochleae were fixed with 2.5% glutaraldehyde and processed for transmission electron microscopy.RESULTS: Nanoparticles were found in cochleae with or without exposure to magnet forces appearing in the RWM, perilymph, endolymph, and multiple locations in the organ of Corti. Electron energy loss spectroscopy confirmed iron elements in nanoparticles.CONCLUSION: The nanoparticles were distributed throughout the inner ear after application on the chinchilla RWM, with and without magnetic forces.SIGNIFICANCE: PLGA-NP applied to the RWM may have potential for sustained therapy to the inner ear. (c) 2007 American Academy of Otolaryngology-Head and Neck Surgery Foundation. All rights reserved.