Comparison of enzyme-linked immunosorbent assay and haemagglutination inhibition test for the detection of antibodies against Newcastle disease virus in ostriches (Struthio camelus)
Comparison of enzyme-linked immunosorbent assay and haemagglutination inhibition test for the detection of antibodies against Newcastle disease virus in ostriches (Struthio camelus)
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DOI：
10.1080/03079459708419218
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发表时间：
1997-06-01
期刊：
影响因子：
2.8
通讯作者：
Zulu, T
中科院分区：
文献类型：
--
作者：
Cadman, HF;Kelly, PJ;Zulu, T
用酶联免疫吸附试验（ELISA）和血凝抑制试验（HI）比较了鸵鸟血清对纽卡斯尔病毒（NDV）的反应性。在第0天和第11天，对两种试验均为血清阴性的10月龄鸵鸟接种油基NDV疫苗。在第11天通过ELISA（11/20只鸟中样品/阳性比率> 0.2; 55%）和m（10/20只鸟中滴度> 1/8; 50%）首次检测到显著水平的反应性抗体。实验结束时（第37天），通过ELISA检测，所有鸟类均具有显着的抗体水平，但通过HI检测，仅16/20（80%）。ELISA法与HI法检测的抗体水平呈S形关系（r = 0.62，三次多项式）。从津巴布韦和博茨瓦纳自然暴露的鸵鸟中也检测到与NDV反应的抗体（112/165; 68%）和HI（85/165; 52%）。
Reactivity of ostrich sera to Newcastle disease virus (NDV) by enzyme-linked immunosorbent assay (ELISA) and haemagglutination inhibition (HI) test were compared. Ten-month old ostriches seronegative by both tests were vaccinated with an oil-based NDV vaccine on days 0 and 11. Significant levels of reactive antibodies were first detected on day 11 by ELISA (sample/positive ratio > 0.2 in 11/20 birds; 55%) and m (titre > 1/8 in 10/20 birds; 50%). At the end of the experiment (day 37) all birds had significant antibody levels by ELISA, but only 16/20 (80%) by HI test. There was a sigmoidal relationship (r = 0.62, 3rd degree polynomial) between antibody levels detected by ELISA and by HI test. Antibodies reactive with NDV in naturally exposed ostriches from Zimbabwe and Botswana were also detected by ELISA (112/165; 68%) and HI (85/165; 52%).