Genetic structure of Mediterranean populations of the sandfly Phlebotomus papatasi by mitochondrial cytochrome b haplotype analysis
Genetic structure of Mediterranean populations of the sandfly Phlebotomus papatasi by mitochondrial cytochrome b haplotype analysis
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DOI：
10.1111/j.1365-2915.2007.00695.x
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发表时间：
2007-09-01
影响因子：
1.9
通讯作者：
Schoenian, G.
中科院分区：
文献类型：
--
作者：
Hamarsheh, O.;Presber, W.;Schoenian, G.
Papatasi（Scopoli）（双翅目：蝇科）是主要利什曼原虫Yakimoff & Schokhor的主要媒介;这是在旧世界自限性皮肤利什曼病的原因。这种白蛉可以在房屋、动物收容所、洞穴和啮齿动物的洞穴中找到。它的地理范围很广，包括中东和地中海地区。对来自10个国家的25个种群的帕帕塔西螨进行了种群分析。分析了番木瓜母系遗传线粒体细胞色素B基因单倍型的分布，以探讨番木瓜群体分化的可能性。通过对该基因3'端442个碱基对的序列比对，从131只白蛉中鉴定出21种单倍型和33个分离位点。序列变异的模式不支持种复合体的存在。中位连接网络方法被用来描述单倍型的起源和人口结构;单倍型往往聚类的地理位置，这表明一定程度的遗传分化的人口之间。我们的研究结果表明，来自叙利亚，土耳其，巴勒斯坦，以色列，约旦和埃及的人口存在显着的人口分化。种群分化的知识，P. papatasi种群是重要的，了解在这个物种的扩散模式，并制定适当的控制措施。
Phlebotomus papatasi (Scopoli) (Diptera: Psychodidae) is the main vector of Leishmania major Yakimoff & Schokhor; which is the cause of self-limiting cutaneous leishmaniasis in the Old World. This sandfly is found in houses, animal shelters, caves and rodent burrows. It has a large geographical range, which includes the Middle East and the Mediterranean regions. A population analysis of colony and field specimens of P. papatasi was conducted on 25 populations originating from 10 countries. The distribution of haplotypes of the maternally inherited mitochondrial cytochrome b gene were analysed to assess the population differentiation of P. papatasi. Alignment of a 442-basepair region at the 3' end of the gene identified 21 haplotypes and 33 segregating sites from 131 sandflies. The pattern of sequence variations did not support the existence of a species complex. The median-joining network method was used to describe both the origin of the haplotypes and the population structure; haplotypes tended to cluster by geographical location, suggesting some level of genetic differentiation between populations. Our findings indicate the presence of significant population differentiation for populations derived from Syria, Turkey, Palestine, Israel, Jordan and Egypt. Knowledge of population differentiation among P. papatasi populations is important for understanding patterns of dispersal in this species and for planning appropriate control measures.